Journal of Genetics and Molecular Biology

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Research Article - Journal of Genetics and Molecular Biology (2019) Volume 3, Issue 2

Composition and molecular weight distribution of albumin and globulin protein isolates from durum wheat genotypes.

This paper attempts to evaluate the banding patterns of non-gluten protein isolates from the grain of durum wheat varieties. Under reduced condition, polyacrylamide-gel electrophoresis has revealed a number of different sized albumin and globulin protein bands. The electrophoretic pattern of globulin showed more polymorphisms than that of albumin. High polymorphism, both in band intensity and occurrence, was observed between 15 kDa and 35 kDa. Most of the protein bands were scored in the range of 10 kDa and 85 kDa in the two protein fractions. At a cut-off point 2.5, cluster analysis based on the SDS-PAGE of globulin proteins classified the durum wheat varieties into three major family-groups. Generally, the experiment showed the suitability and usefulness of globulin protein fractions as a genetic marker in discriminating durum wheat genotypes.

Author(s): Hailegiorgis D, Lee CA, Yun SJ*

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