Biomedical Research

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Research Article - Biomedical Research (2020) Volume 31, Issue 6

Antimicrobial effectiveness of Chamaecyparis obtuse extracts for intracanal bacteria removal using Enterococcus faecalis

This study aimed to investigate the antimicrobial activity of Chamaecyparis obtusaextract on Enterococcus faecalis (E. faecalis), typical causative bacteria that causes endodontic treatment failure and pulpitis. Chamaecyparis obtusa was immersed in 70% ethanol for 12 h, and concentrated Chamaecyparis obtusa extract was applied to E. faecalis diluted to 1 × 106 CFU/ml at the 0, 1.25, 2.5, 5, 10, 20, and 30 mg/ml concentrations. Then the colony-forming units (CFUs) were checked at 24 h to evaluate the antimicrobial activity of the extract. The minimal inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were checked to evaluate the antibacterial effect. The CFUs and survival rate of E. faecalis showed growth inhibition as the concentration increased. The MIC and MBC were found to be 2.5 and 30 mg/mL, respectively. Chamaecyparis obtusaextract with an antimicrobial effect on E. faecalismay be used as an irrigation agent in place of chemicals that cause resistance in endodontic treatment.

Author(s): Seoul-Hee Nam, Yu-Rin Kim

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