Research Article - Journal of Plant Biotechnology and Microbiology (2018) Volume 1, Issue 1
Graphene oxide inhibits the lethal browning of Cymbidium sinense by reducing activities of enzymes.
The major problems associated with plant tissue culture is browning of the culture medium, which invariably leads to decreased regenerative ability, poor growth and even death of the culture tissue. Here we show that the browning of Chinese orchid tissue culture was inhibited efficiently by graphene oxide. Our results demonstrate that the effect of the GO was much more effective than that of the activated charcoal (AC), resulting GO much more markedly promoted bud growth, reduced the browning index and alleviated the effect of browning during bud differentiation and development than AC. We found GO alleviated the browning by reducing the activities of enzymes like PPO and POD, and possibly by adsorbing quinones compounds. We anticipate our assay to be a starting point for more efficient plant tissue culture, and wildly used in agriculture, forestry, medicine, industry, and etc, producing a huge economic and social benefits.Author(s): Ting Wu, Juan Li, Jinglin Zhang, Minsong Lin, Zhimin Wu, Xiang Cai, Weibin Xiang, Herong Guo, Shaozao Tan, Zhisheng Zhang