Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +1-504-608-2390

Research Article - Biomedical Research (2021) Volume 32, Issue 3

Beneficial effect of fasudil in osteoarthritis by enhancing the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells

Present report evaluates the effect of fasudil on the chondrogenic differentiation of BMSCs and on management of OA (in vivo and in vitro). Primary BMSCs were isolated from each rat for in vitro study and BMSCs were treated in presence and absence of fasudil. Cell viability assay was assessed by CCK-8 assay and chondrogenic differentiation and migration of BMSCs by incubating with fasudil for 14 days. In vivo OA was induced in rats by surgical procedure and treated with fasudil 30 mg/kg, p.o. Level of cytokines and expression of JNK, ERK and p38 protein were assessed in the cartilage tissue of OA rats. There was significant increase in the cell viability and migration in fasudil 100 nM treated BMSCs than fasudil 0 nM. Protein and mRNA expression of Sox9, Col2a1, Aggrecan and Col10a1 were significantly enhanced in fasudil treated 10 and 100 nM compared to 0 nM on day 7 and 14. Expression of JNK, p38 and ERK protein was reduced significantly (P<0.01) in the fasudil treated group than control group of BMSCs. The fasudil reduced cytokine level and TGF-β, and increased the expression of MMP-13, Col II in the cartilage tissue of OA rats. Moreover, treatment with fasudil ameliorates the histopathological changes in the cartilage tissue of OA rats. In conclusion, fasudil treatment protects the osteoarthritis by promoting the chondrogenic differentiation of bone marrow-derived mesenchymal stem cells. Moreover, fasudil reduces the level of cytokine and activation of JNK/MAPK/ERK signaling pathways in the cartilage tissue of OA rats.

Author(s): Nan Min, Jie Ma, Lei Shi, Lin Wang, Chi Liu, Yaonan Zhang, Qingyun Xue

Abstract Full Text PDF

Get the App

best replica reviews