Biology & Medicine Case Reports

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Biology & Medicine Case Reports 44 7897 074717

Size Exclusion Chromatography Innovations

A novel method for analysing polysaccharide materials is described which employs size‐exclusion chromatography (SEC) followed by detection by on‐line electrospray ionisation mass spectrometry (ESI‐MS) and off‐line matrix‐assisted laser desorption/ionisation time‐of‐flight mass spectrometry (MALDI‐TOFMS). it's demonstrated through SEC/ESI ion trap mass spectrometry that the formation of multiply charged oligomer ions, which bind up to 5 sodium cations, allows the rapid analysis of polysaccharide ions with molecular weights in more than 9 kDa. MALDI spectra generated from fractionation of the effluent collected from an equivalent SEC separation are shown to be in good agreement with the ESI spectra with reference to relative molecular mass distributions and kinds of ions generated. ESI and MALDI mass spectra of samples obtained from sequential graded ethanol precipitation and SEC fractionation of acid and enzymatically digested arabinoxylan polysaccharides show important structural differences between polysaccharide fragments. additionally , a comparison is formed between the mass spectra of native and permethylated SEC‐separated fragments of acid and enzymatically treated arabinogalactan. Linkage information of the permethylated arabinogalactan oligomers are often rapidly established through the utilization of on‐line SEC/ESI‐MS n experiments.

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