Journal of Gastroenterology and Digestive Diseases

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Molecular Detection

Molecular detection of biological agents within the field has historically relied on the employment of quantitative time period PCR (qPCR), that currently includes commercially out there instruments which will be employed in the laboratory or field. Adapting this technology for field-forward applications necessitated innovation to reduce size, weight, and power needs. Rugged, transportable instruments, economical power sources, freeze-dried reagents, information communications, and customary in operation procedures for minimally trained users area unit some samples of limitations that are overcome to permit qPCR-based information to be generated at the purpose of would like. Despite the high specificity and sensitivity of qPCR, the assays need a priori sequence-based information of the etiological agent to style and turn out specific targeted assays with primers and probes.

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