Asian Journal of Biomedical and Pharmaceutical Sciences

Asian Journal of Biomedical and Pharmaceutical Sciences 44 7897 074717

Immobilisation Peer-review Journals

An immobilized enzyme is an enzyme attached to an inert, insoluble material—such as calcium alginate (produced by reacting a mixture of sodium alginate solution and enzyme solution with calcium chloride). This can provide increased resistance to changes in conditions like pH or temperature. It also lets enzymes be held in situ throughout the reaction, following which they're easily separated from the products and should be used again - a much more efficient process then is widely utilized in industry for enzyme catalysed reactions. An alternative to enzyme immobilization is whole cell immobilization.

Adsorption on glass, alginate beads or matrix: Enzyme is attached to the surface of an inert material. In general, this method is that the slowest among those listed here. As adsorption isn't a reaction, the site of the immobilized enzyme could also be blocked by the matrix or bead, greatly reducing the activity of the enzyme. Entrapment: The enzyme is trapped in insoluble beads or microspheres, like calcium alginate beads. However, these insoluble substances hinder the arrival of the substrate, and therefore the exit of products.

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