Journal of Plant Biotechnology and Microbiology

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Micropropagation

Micropropagation is a tissue culture technique used for rapidly multiplying stock plant material to produce large number of progeny plants and then planting them out. The four major steps to carry out this process are (a) Initiation stage wherein a plant tissue is taken, disinfected and placed on a medium (b) Multiplication stage (c) Rooting stage (d) Acclimatization. Microropagation differs from tissue culture as micropropagation must use tissue culture to create a new plant whereas tissue culture can create a plant directly. Plants grown by this method will be genetically identical as that of the original plant from where they were grown. Micropropagation techniques are of three types categorized based on the way of propagation, (a) Propagation from shoot (b) multiple shoot differentiation (c) Embryo differentiation.

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