Allied Journal of Medical Research

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Allied Journal of Medical Research 44 7897 074717

Importance Of Lipidomics

Typical lipidomics data are obtained from collective cell lysis encompassing the digestion of
large groups of cells or tissues, resulting in inaccuracies and averaging of sample data. Here wedescribe a completely unique approach of obtaining single cell information with results demonstrating theimportance of single cell analysis. We report obtaining distinct heterogeneity of adipocytes in vitro using nanomanipulation-coupled nanoelectrospray mass spectrometry of living individual cells from an equivalent culture plate.

There is an excellent need for single cell analysis for chemical profiling of biological materials in order to create an accurate model of cellular function. Currently, the standard application of obtaining a chemical profile of biomaterials consists of mechanical or chemical digestion of
large groups of cells. Following digestion, organic extractions are performed to get the targeted biochemistry to be used in analysis like western blots , chromatography , and electrospray ionization mass spectrometry (ESI-MS).
The inherent problem with these multiple cell analysis methods is that the info obtained is representative of an ensemble average of the population, which may obscure variations within individual cells comprising the population.
In addition, the whole sample of cells analyzed are sacrificed within the process and can't be
monitored afterwards, which is vital surely progressive diseases like cancer. It has been established that individual cells and their lipid contents aren't homogenous, so it's imperative to review them at the only cell level to raised understand lipidomic processes and cellular functions.

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