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J u l y 2 3 - 2 4 , 2 0 1 8 | R o m e , I t a l y
allied
academies
Joint Event on
Cardiology Congress 2018 & Microbe Infection 2018
Biomedical Research
|
ISSN: 0976-1683
|
Volume 29
2
nd
World Congress on
CARDIOLOGY
MICROBIOLOGY AND MICROBIAL INFECTION
&
39
th
Annual Congress on
Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C1-003
HIGH-THROUGHPUT CARBON SUBSTRATE PROFILING OF
MYCOBACTERIUM ULCERANS
SUGGESTS POTENTIAL ENVIRONMENTAL
Dezemon Zingue
1,2
, Amar Bouam
2
, Muriel Militello
2
and
Michel Drancourt
2
1
Centre MURAZ, Burkina Faso
2
Aix Marseille Université, France
Background:
Mycobacterium ulcerans
is a close derivative of
Mycobacterium marinum
and the agent of buruli ulcer in some
tropical countries. Epidemiological and environmental studies pointed towards stagnant water ecosystems as potential sources
of
M. ulcerans
, yet the ultimate reservoirs remain elusive. We hypothesized that carbon substrate determination may help
elucidating the spectrum of potential reservoirs.
Methodology & Results:
In a first step, high-throughput phenotype microarray BIOLOG was used to profile carbon substrates in
one
M. marinum
and five
M. ulcerans
strains. A total of 131/190 (69%) carbon substrates were metabolized by at least one
M.
ulcerans
strain, including 28/190 (15%) carbon substrates metabolized by all five
M. ulcerans
strains of which 21 substrates were
also metabolized by
M. marinum
. In a second step, 131 carbon substrates were investigated, through a bibliographical search,
for their known environmental sources including plants, fruits and vegetables, bacteria, algae, fungi, nematodes, mollusks,
mammals, insects and the inanimate environment. This analysis yielded significant association of
M. ulcerans
with bacteria
(p=0.000), fungi (p=0.001), algae (p=0.003) and mollusks (p=0.007). In a third step, the Medline database was cross-searched for
bacteria, fungi, mollusks and algae as potential sources of carbon substrates metabolized by all tested
M. ulcerans
; it indicated
that 57% of
M. ulcerans
substrates were associated with bacteria, 18% with alga, 11% with mollusks and 7% with fungi.
Conclusions:
This first report of high-throughput carbon substrate utilization by
M. ulcerans
would help designing media to
isolate and grow this pathogen. Furthermore, the presented data suggest that potential
M. ulcerans
environmental reservoirs
might be related to micro-habitats where bacteria, fungi, algae and mollusks are abundant. This should be followed by targeted
investigations in buruli ulcer endemic regions.
zinguedezemon@yahoo.fr