cardiology-congress-microbe-infection-2018-posters

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J u l y 2 3 - 2 4 , 2 0 1 8 | R o m e , I t a l y

Note:

allied

academies

Joint Event on

Cardiology Congress 2018 & Microbe Infection 2018

Biomedical Research

ISSN: 0976-1683

Volume 29

World Congress on

CARDIOLOGY

MICROBIOLOGY AND MICROBIAL INFECTION

Annual Congress on

Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C1-003

THE EFFECT OF POMEGRANATE EXTRACT ON SURVIVAL AND

PERITONEAL BACTERIAL LOAD IN CECAL LIGATION AND PERFORATION

MODEL OF SEPSIS RAT

Shahryar Eghtesadi, Sanaz Tavasoli, Amir-Hassan Zarnani, Mohamadreza Vafa, Maziar Moradi-Lakeh, Alire-

za Sadeghipour

and

Maryam Eghtesadi

Azad University, Iran

epsis is one of the major causes of death in intensive care units. Oxidative stress and hyper-inflammation has been shown

to be major cause of mortality and morbidity in septic cases. Pomegranate is a fruit which is considered for its antioxidant

and anti-inflammatory properties. The aim of this study was to evaluate the effect of POMx, a standard pomegranate extract,

on mortality and peritoneal bacterial load in cecal ligation and perforation (CLP) model of sepsis in rats. Male Wistar rats were

divided into four groups: sham; CLP; prevention [consumed POMx (250 mg of polyphenols/kg/day) for four weeks and subjected

to CLP]; treatment [subjected to CLP and then received a single drink of POMx (250 mg of polyphenols/kg)]. Sepsis was induced

by CLP surgery. 10 days survival rate of all groups (subdivided into with and without antibiotics subgroups) were recorded.

Peritoneal bacterial load of animal was also assessed. Data were analysed using log-rank and Kruskal-Wallis tests. There were

no significant differences in survival rates of CLP, prevention and treatment groups, in subgroups without antibiotics. However,

in subgroups with antibiotics, the prevention group had significantly lower survival rate than sham group (p<0.05). Conversely,

the bacterial load of prevention and treatment group were significantly higher than sham group (p<0.01). In conclusion, our

study demonstrated that pomegranate extract could increase mortality rate via increasing peritoneal cavity bacterial load, in CLP

model of sepsis. More studies to assess mechanisms of this effect are warranted.