cardiology-congress-microbe-infection-2018-posters

Page 48

J u l y 2 3 - 2 4 , 2 0 1 8 | R o m e , I t a l y

allied

academies

Joint Event on

Cardiology Congress 2018 & Microbe Infection 2018

Biomedical Research

ISSN: 0976-1683

Volume 29

World Congress on

CARDIOLOGY

MICROBIOLOGY AND MICROBIAL INFECTION

Annual Congress on

Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C1-003

LACTOBACILLUS CASEI

COULD BE A BIO-THERAPEUTIC FOR ENTERIC

BACTERIAL INFECTIONS

D Biswas

and

M Peng

University of Maryland, USA

s a major source of microbes and their numerous beneficial effects, the gut microflora/microbiome is intimately linked

to human health, immunity and diseases. The key intestinal microbial byproducts, commonly known as metabolites, are

crucial to the maintenance of a balanced gut ecosystem and healthy gut microbial community. More specifically, the presence or

absence of several genes and their expression levels, in the presence or absence of stimuli or stress, regulate the production and

concentration/number of various metabolites. These are essential for host defense and immunity and protecting from various

diseases or pre-condition of diseases including inflammation, cancer, oxidation, atherosclerosis, and out competition of enteric

bacterial pathogens. In a recent study, we found that in the presence of the prebiotic-like component peanut flour,

Lactobacillus

casei

(LC) produced 100 times more linoleic acid (LA) than under normal conditions and was able to outcompete several enteric

bacterial pathogens. Based on this evidence, we have overexpressed the linoleate isomerase (myosin cross-reactive antigen,

mcra

) gene in a natural, sustainable, bacteriophage-resistant LC strain (LC

+mcra

) to enhance the production of conjugated linoleic

acids (CLA) and verify the ability of this genetically engineered strain LC+mcra to inhibit growth, colonization, and infection

of host cells by human enteric foodborne bacterial pathogens. We found that LC

+mcra

excluded the Salmonella and EHEC in

co-culture condition and altered the host cell-pathogens (both) interactions. The genetically modified mutant also altered the

virulence properties of both bacterial pathogens significantly. This study showed that LC

+mcra

could be a non-traditional bio-

therapeutic for preventing the colonization of

Salmonella

and EHEC.