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J u l y 2 3 - 2 4 , 2 0 1 8 | R o m e , I t a l y
Note:
alliedacademies Joint Event on
Cardiology Congress 2018 & Microbe Infection 2018 Biomedical Research | ISSN: 0976-1683 | Volume 29
2nd World Congress on CARDIOLOGY
MICROBIOLOGY AND MICROBIAL INFECTION
&
39th Annual Congress on
Bikram Gautam et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C1-003
HEAVY METAL AND ANTIBIOTIC RESISTANT
BACTERIA ISOLATED FROM GUHESWORI
SEWAGE TREATMENT PLANT, NEPAL
Bikram Gautam1,2 and Rameshwar Adhikari2
1St. Xavier’s College, Nepal
2Tribhuvan University, Nepal
The aim of this project is to simply determine the resistance level of
microorganisms isolated from the treated sewage effluent. To accomplish
this first their resistance pattern against antibiotics and heavy metals were
screened as per Clinical and Laboratory Standards Institute, 2017 and
British pharmacopeia, 2016 respectively. This study also provides evidence
that these microorganisms have metabolized the heavy metals and hence
might be very useful in tackling problems of metal poisoning due to both
spillage and/or due to geographical location. Sewage contains chemicals
(biocides, detergents, heavy metals etc.) and microorganisms. Bacteria get
exposed to the chemicals and acquire resistance to antibiotic(s) and heavy
metal(s). The aim of this study was to the isolated bacteria from the treated
sewage and assess resistance pattern of the isolates against antibiotics and
heavy metals. Grab sampling was performed from the treated effluent at
the Guheswori sewage treatment plant. To assess the resistance pattern for
antibiotic(s) and heavy metal(s), antibiotic susceptibility test and minimum
inhibitory concentration by cup well method were performed as per Clinical
and Laboratory Standards Institute, 2017 and British pharmacopeia, 2016
respectively. Staphylococcus aureus, Enterococcus faecalis, Citrobacter
freundii, Escherichia coli, Enterobacter aerogenes, Proteus mirabilis, P.
vulgaris, Salmonella Typhi, Pseudomonas aeruginosa were isolated from the
treated sewage. Multi drug resistant and heavy metal resistant isolates were
screened. P. aeruginosa was able to resist the heavy metal concentration up
to 10000 g/L dilution of Fe++. Pearson’s chi square test shows that there is a
significant association (p<0.001) between isolates and antibiotic resistance
pattern; isolates and heavy metal resistance pattern at dilution 10000 g/L,
5000 g/L, 2500 g/L, 1250 g/L. The findings of the study show that the sewage
treatment plant is not capable of effluent polishing. The isolates from treated
sewage were found to be resistant to multiple heavy metals and antibiotics.
Heavy metal resistant bacteria could be utilized in lower metal pollution
through bio-absorption, mineralization, enzymatic oxidation/reduction to a
less toxic form etc.
Bikram Gautam is a fourth semester MSc Microbiolo-
gy student at Department of Microbiology, St. Xavier’s
College, Maitighar, Nepal. He has published three re-
search papers to date. He is serving as a Research As-
sistant at RECAST and working as a Quality Controller
at Senior Shree Krishna Beverage, Kathmandu, Nepal.
BIOGRAPHY