Allied Journal of Medical Research

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Allied Journal of Medical Research 44 7897 074717

Size Exclusion Chromatography Open Access Articles

Size exclusion chromatography is that the method of choice for separating free from liposome-encapsulated molecules. However, if the column isn't presaturated with lipids this sort of chromatography causes a big loss of lipid material. To date, the mechanism of lipid retention is poorly understood. it's been speculated that lipid binds to the column material or the whole liposome is entrapped inside the void.
Here we show that intact liposomes and their contents are retained within the exclusion gel. Retention depends on the pore size, the smaller the pores, the upper the retention. Retained liposomes aren't tightly fixed to the beads and are slowly released from the gels upon direct or inverted eluent flow, long washing steps or column repacking. Further addition of free liposomes results in the elution of a part of the gel-trapped liposomes, showing that the retention is transitory. Trapping reversibility should be associated with a mechanism of partitioning of the liposomes between the stationary phase, water-swelled polymeric gel, and therefore the mobile aqueous phase.
Retention of liposomes by size exclusion gels may be a dynamic and process , which should be accounted for to regulate lipid loss and sample contamination during chromatography

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