+1 (202) 780-3397THE HISTOPATHOLOGICAL STUDY OF “HORSE BONE CALCIUM” PREPARATION EFFECT ON THE ACETIC INDUCED GASTRIC ULCER MODEL
Joint Event on 4th International Conference on GASTROENTEROLOGY AND HEPATOLOGY & 9th World Congress on CLINICAL PHARMACY & PHARMACY PRACTICE
March 25-26, 2019 | Amsterdam, Netherlands
B Myagmarnaran, M Ariunaa1, Z Ariunaa, D Аdilzaya, B Altanshagai and D Demberelnyamba
Science, Technology & Production “Monchemo” Co. Ltd., Mongolia Treatment, Research and Production Company of The Mong-Em, Mongolia Mongolian National University of Medicinal Sciences, Mongolia Mongolian University of Pharmaceutical Science, Mongolia New Medicine Medical University of Mongolia, Mongolia
Scientific Tracks Abstracts : Arch Gen Intern Med
Abstract:
Introduction: Calcium homeostats is: Horse bone calcium supplement contains calcium hydroxyapatite,
which is in the form of complex chelates ion, gives effect of diminish gastric acid and pepsin. Calcium hydroxy-
apatite is crystallizes in the hexagonal crystal system. Size of the calcium hydroxy-apatite nano particles
are 105-120 nm.
Microcrystalline hydroxyapatite concentrate (MCHC) is derived from whole bone and is available as a nutritional
preparation. It provides much greater nourishment than just calcium. MCHC contains protein and other
ingredients that comprise the organic portion of bone, as well as calcium and other minerals in the normal
physiological proportions found in raw bone.
There is no doubt that calcium is essential for healthy bone formation; however, trace minerals and organic
factors are also important. Because bone is a complex, highly mineralized tissue, a number of trace mineral deficiencies
can impair bone formation and remodeling. Trace minerals also act as cofactors for several enzymes
involved in the production of the organic portion of bone. Because MCHC is actual bone, it contains these
vital components, which are important for a healthy skeleton. It truly is comprehensive bone nourishment. No
stress for gastric digestion system.
Materials and methods: The effects of the medicinal substances were investigated on “Wistar” breed of white
rats. Many studies mostly experimental animal effect have been conducted in thus field. In this study, we examined
gastroprotection effect of “HORSE BONE CALCIUM” preparationon the gastric ulcer in rats model induced
by acetic acid. The stomach exposed and 0.02 ml of 100% acetic acid was injected into the gastric wall near
the antral portion of the stomach using a hamilton syringe with a 30-gauge needle. Intact animals received no
surgical modifications. The abdomen was then sutured, and the animals were allowed to recover and returned
to their cages with food and water ad libitum. Injection of 0.02 ml of acetic acid causes. The stomach protective
process starts around day 7 and is completed in about 4 weeks.
The animals were divided into 3 groups and treated daily by gavage with“HORSE BONE CALCIUM” in 1.2 ml of
solvent. At the end of 4 weeks, the animals were killed and their stomachs removed, opened along the greater
curvature, fixed in 10% neutral buffered formalin overnight, and photographs taken.
Results: Histopathological studies: Freshly excised stomach of one animal from each group was washed with saline and preserved in 10% formaldehyde solution for hystopathological studies. It was processed for 12 h
using isopropyl alcohol, xylene and paraffin embedded for light microscopic study. Paraffin embedded tissue
section cut at 5 μm thickness were prepared and stained after deparaffination using hematoxylene and eosin
stain (H and E) to verify morphological assessment of stomach damage. Photomicrographs were captured at
a magnification of 10, 20 X.
“HORSE BONE CALCIUM”preparation said the ulcer by reducing the epithelium and epithelial tissue of the gastric
mucosa of experimental animals.
Conclusion:
1. Gastric ulcer model has been developed as pathohystological method to the experimental and control
animal groups.
2. “HORSE BONE CALCIUM” supplement exhibited a gastroprotective effect against gastric injury induced
by acetic acid in rats.
Biography:
B Myagmarnaran is a pharmacist, researcher of science, technology & production at Monchemo, Mongolia. She has been working at Monchemo as a researcher since 2014 when she was a bachelor’s degree student of Mongolian University of Pharmaceutical Science. In 2018, She has been working as a quality control manager and researcher in treatment, research and production company of “Mong-em”. Also, she has been studying master’s degree course at Mongolian University of Pharmaceutical Science since 2018.
E-mail: myagmarnaran.b@yahoo.com
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