Salmonella Typhimurium and Salmonella Enteritidis infections in sporadic diarrhea in children: Source tracing and resistance to third-generation cephalosporins and ciprofloxacin
Joint Event on 7th European Clinical Microbiology Congress & 4th International Conference on Ophthalmology and Eye Disorder
November 01-02, 2018 | London, UK
Childrens Hospital of Fudan University, China
Posters & Accepted Abstracts : Clinical Microbiology and Eye
Objectives: This study is aimed to trace the transmission source of Salmonella Typhimurium and Salmonella Enteritidis strains associated with enteric infections in Shanghainese children, and understand the molecular mechanism of resistance to thirdgeneration cephalosporins and ciprofloxacin. Methods: The profiles of pulsed-field gel electrophoresis (PFGE) were compared among the isolates from children, animal and environment. Antimicrobial susceptibility was determined using the minimal inhibitory concentrations and Kirby-Bauer disk diffusion method. Genes mediating extended-spectrum β-lactamase (ESBL) producing were identified using PCR and sequencing. Results: Based on PFGE patterns, 49 (33.1%) of 148 human Salmonella Typhimurium isolates in the dominant PFGE clusters were genetically related to the isolates from poultry source, environment water, aquatic products and reptiles and 97(97.0%) of 100 human Salmonella Enteritidis isolates were genetically related to isolates from poultry and water. The rates of resistance to ceftriaxone among clinical Salmonella Typhimurium and Salmonella Enteritidis isolates were 42.0% and 14.2%, respectively. Besides, 35.1% of clinical Salmonella Typhimurium isolates were resistant to ciprofloxacin. The rates of resistance to cefotaxime and ciprofloxacin among Salmonella Typhimurium isolates from freshwater food animals and Salmonella Enteritidis isolates from pork meat were 13% and 69.6%, and 18.2% and 9.1%, respectively. Of the 64 ESBL/AmpCproducing strains, CTX-M, TEM, DHA and CMY were found in 86.0%, 62.5%, 7.8%, 3.1% and 3.1% of isolates, respectively. Conclusions: The transmission sources of Salmonella Typhimurium and Salmonella Enteritidis infections in Shanghainese children were diverse. The high prevalence of resistance to third-generation cephalosporins and ciprofloxacin mediated by multiple molecular mechanisms need continuous attention and intervention.