+441518081136Phytochemical composition, cytotoxicity and in-vitro antiplasmodial activity of fractions from Alafia barteri olive (Hook F. Icon)-Apocynaceae
4th International Congress on Drug Discovery, Designing and Development & International Conference and Exhibition on Biochemistry, Molecular Biology: R&D
November 02-03, 2017 Chicago, USA
Lasisi A A, Olayiwola M A, Balogun S A, Akinloye O A and Ojo D O
Federal University of Agriculture, Nigeria Federal College of Education, Nigeria
Posters & Accepted Abstracts : J Pharmacol Ther Res
Abstract:
The ethanolic extract of Alafia barteri (Hook F. Icon) was dissolved in distilled water and successively partitioned in n-hexane, chloroform, ethyl acetate and n-butanol. The fractions were evaluated for phytochemical composition, lethality against brine shrimp larvae and in-vitro antiplasmodial activity against Plasmodium falciparum strain. The obtained results revealed that the roots and leaf extracts of A. barteri exhibited broad spectrum of antiplasmodial activity (IC50 1.5±0.7 - 6.2±0.80 µg/mL). The aqueous leaf fractions displayed the most potent antiplasmodial activity with an IC50 value of 1.5±0.7 µg/mL, which is comparable to reference antimalarial drug (IC50 value of 1.3±0.2 µg/mL). The leaf fractions displayed higher activity than the root extracts. The highest minimum lethal concentration (105.2±0.8 ppm) was exhibited by the aqueous leaf extract followed closely by the root extract (120.2±1.1 ppm). The leaf extracts contained higher polyphenols (45.3±0.85 mgGAE/g) and flavonoids (18.10±0.2 mgCTE/g) than the root extracts. The n-hexane and EtOAc extracts/fractions displayed lower activity on brine shrimp larvae.
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