OPTIMIZING THE SEED GERMINATION OF GARCINIA MANGOSTANA L. THROUGH PRIMING TREATMENTS
World Congress on PLANT GENOMICS AND PLANT SCIENCE
November 23-24 , 2018 | Bangkok ,Thailand
Puzon Juliana Janet M
University of the Philippines, Philippines
Scientific Tracks Abstracts : J Agric Sci Bot
Garcinia mangostana L., commonly known as mangosteen, has recalcitrant seeds that remain dormant unless exposed to optimal environmental conditions. In various crops, seed priming treatments are known to enhance seed germination, and alter the seed’s metabolic activity before germination. The study aimed to optimize the germination of G. mangostana seeds through different priming treatments, namely, soaking the seeds in H2O, H2O2 and acid scarification with HNO3. The effectiveness of the priming treatments in inducing seed germination was compared. The lipid and carbohydrate components of the seeds that had undergone priming were determined. Mangosteen seeds were subjected to three different methods of seed priming, namely soaking seeds in distilled water, H2O2 acid scarification with HNO3 before germinating in the dark for seven days. The germination percentage, mean germination time, and germination rate were measured every day. After 7 days of germination, total lipids and total carbohydrates in ungerminated and germinated seeds were quantified using modified Folch method, and Dubois assay, respectively. The results of seed germination showed that soaking the seeds in 0.5% H2O2 was the most favourable among all the priming treatments based on the values of measured germination parameters. A significant difference between the mean % germination of seeds was observed, while there was no significant difference between the mean germination rates and mean germination time of seeds between treatments. Increasing concentrations of H2O2 and HNO3 reduced the lipid content of seeds. The carbohydrate content of the germinated seeds primed with distilled water and increasing concentrations of H2O2 decreased, while seeds primed with increasing concentrations of HNO3 had increasing carbohydrate content. Therefore, the concentrations of H2O2 and HNO3 in the priming treatments greatly affect the lipid and carbohydrate contents of the seeds. Results of this study serve as a significant contribution to the improved propagation of G. mangostana L., an economically valuable species.
Puzon Juliana Janet M is a botany professor in the Institute of Biology, College of Science, University of the Philippines, Diliman, Quezon City. She teaches botany subjects and heads the Plant Physiology Research Laboratory in this institute. Her current research interests include plant stress physiology, phytotechnologies, bioactive secondary metabolites, and physico-chemical and phytohormonal control of seed germination.
E-mail: [email protected]