In vitro anti-leishmanial activity of Artemisia dracunculus and Heracleum persicum extracts in comparison with glucantime
Joint Conference on GLOBAL APPLIED MICROBIOLOGY CONFERENCE & International Congress on MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017 Toronto, Canada
Batool Sadeghi-Nejad, Shahram Khademvatan, Alborz Eskandari and Sedigheh Yusef Naanaie
Abadan School of Medical Sciences, Abadan, Iran Urmia University of Medical Sciences, Iran Jundishapur University of Medical Sciences, Iran The Agricultural and Natural of Resources Center, Iran
Posters & Accepted Abstracts : Microbiology: Current Research
Background & Objectives: Cutaneous leishmaniasis (CL) is one of the most common parasitic diseases. It is one of the major public health in developing countries and throughout the world. Pentavalent antimonial compounds like pentostam and glucantime has been used to treat CL for the last 50 years. The use of these compounds has some limitations such as long duration of treatment, high expenses of drugs, and methods of drug use which are intradermal and intramuscular injection. Beside these, lack of response to the treatment in 10-15% of cases and toxic effects on heart, liver, and kidneys are other possible side effects. Hence, the objective of the present survey was to state the antileishmanial activity of two herbal medicine (Artemisia dracunculus and Heracleum persicum) extracts were evaluated against Leishmania major and Leishmania infantum using colorimetric MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5diphenyl-2H-tetrazolium bromide) assay and compared to the glucantime as a reference. Materials & Methods: The leaves extracts of selected plants were obtained by maceration. The in vitro assays were carried out on L. major and L. infantum using colorimetric MTT assay in comparison with glucantime. The concentration-response curves tested extracts and glucantime solutions were designed and IC50 values were located. Results: Anti-Leishmina effects of A. dracunculus and H. persicum) on L. major and L. infantum promastigote were revealed with 50% inhibitory concentration (IC50) values of 49.67 and 42.23 mg ml-1 for A. dracunculus, 81.15 and 73.17 mg mg ml-1 for H. persicum. In comparison with the standard drug, glucantime had IC50 value of 40.2 mg ml-1 for L. major and 18.5 mg ml-1 for L. infantum promastigote after 72 hours incubation respectively. Conclusion: These results revealed that compounds from Satureja khuzestanica and Heracleum persicum have antileishmania properties that necessary to survey the effects of these extracts on leishmania genus in animal models in future.
Batool Sadeghi-Nejad is working at Abadan School of Medical Sciences, Abadan, Iran. She has published many research papers.