Journal of Clinical and Experimental Toxicology

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Genotoxic and mutagenic evaluation of graphene oxide

Webinar on 23rd Euro-Global Summit on Toxicology and Applied Pharmacology & 18th International Conference on Metabolomics & Systems Biology
March 09-10, 2022 WEBINAR

Angeles. Jos*, Oscar Cebadero-Dominguez, C Medrano-Padial, M Puerto and A M Camean

University of Seville, Spain

Scientific Tracks Abstracts : J Clin Exp Tox

Received:   03/02/2022   Accepted:   07/02/2022   Published:   09/03/2022


Graphene oxide (GO) is a graphene derivative used in many different fields. Compared with other carbon materials, GO could provide advantages for biomedical applications. But any potential use will depend on its safety, and genotoxicity and mutagenicity aspects play a key role in any toxicological evaluation. Thus, the aim of this work was to explore the genotoxicity of GO by the Micronucleus (MN) test (OECD 487) and the Comet assay. Mutagenicity was evaluated using the Mouse lymphoma assay (MLA, OECD 490). A lymphoma cell line (L5178Y Tk+/-) was used for MN and MLA and the human colorectal adenocarcinoma cell line (Caco-2) for the comet assay. 250 μg/mL GO was used as the highest concentration based on previous cytotoxicity studies. Regarding the MN test, GO did not increase the number of binucleated cells at any concentration assayed. No DNA damage was observed in Caco-2 cells treated with GO after 24h and 48h. For MLA, there was no mutagenic effect after both exposure times at any concentration assayed. We can conclude that GO is neither genotoxic nor mutagenic at the conditions tested, but further toxicological tests are required. Acknowledgement: Project US-1259106 cofunded by Programa Operativo FEDER 2014-2020 and Consejería de Economía, Conocimiento, Empresas y Universidad de la Junta de Andalucía. And project P18-RT-1993 (PAIDI-2020, Junta de Andalucía). Biology Services of CITIUS is acknowledged for technical assistance.
Recent Publications
1. O. Cebadero et al., (2022). In vitro toxicity evaluation of graphene oxide and reduced graphene oxide on Caco-2 cells. Toxicology reports.
2. S. More et al., “Guidance on risk assessment of nanomaterials to be applied in the food and feed chain: human and animal health,” EFSA Journal, vol. 19, no. 8, 2021.
3. OECD (2016), Test No. 487: In Vitro Mammalian Cell Micronucleus Test, OECD Guidelines for the Testing of Chemicals, Section 4, OECD Publishing, Paris.
4. OECD (2015), Test No. 490: In Vitro Mammalian Cell Gene Mutation Tests Using the Thymidine Kinase Gene, OECD Publishing, Paris.
5. Azqueta A and Dusinska M (2015) The use of the comet assay for the evaluation of the genotoxicity of nanomaterials. Front. Genet. 6:239.


Ángeles Jos is PhD in Pharmacy at the University of Sevilla. She is Full Professor of Toxicology in the Department of Nutrition and Bromatology, Toxicology and Medicine of this university. Her research focuses on different fields of Toxicology, such as the evaluation of food safety and toxicity of different substances (nanomaterials, food additives, or toxins).

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