Genomic features, novel antibiotic resistance genes and pan-genome analysis of clinical multidrug-resistant Acinetobacter baumannii strains in Vietnam hospital
Joint Event on 7th European Clinical Microbiology Congress & 4th International Conference on Ophthalmology and Eye Disorder
November 01-02, 2018 | London, UK
Thongnhat Dongnai General Hospital, Vietnam
Posters & Accepted Abstracts : Clinical Microbiology and Eye
Background: Acinetobacter baumannii is an important nosocomial pathogen that can develop multidrug resistance. In this study, we sought to explore the genomic properties, phylogenetic relationships and comparative genomics of this pathogen through strain DMS06669 and DMS06670 (isolated from the sputum of two male patients with hospital-acquired pneumonia). Methods: Whole genome analysis of A. baumannii DMS0669 and DMS06670 included de novo assembly; gene prediction; functional annotation to public databases; phylogenetic tree construction by average nucleotide identity; pan-genome analysis and antibiotics resistance genes identification. Antibiotics resistance genes in-vitro were isolated by PCR and re-confirmed by improved Sanger method. Results: The data showed that a total of 19 possible antibiotic resistance genes, conferring resistance to eight distinct classes of antibiotics, were identified in two strains. Nine of these genes have not previously been reported to occur in A. baumannii. Comparative analysis of 23 available genomes of A. baumannii revealed an open pan-genome consisting of 15,883 genes. All antibiotics resistance genes were isolated. Conclusions: Our results provide important information regarding mechanisms that may contribute to antibiotic resistance in the DMS06669 and DMS06670 strain and have implications for treatment of patients infected with A. baumannii.