Functionalization of ZnO nanoparticles by 3-mercaptopropionic acid for aqueous curcumin delivery: Synthesis, characterization and anticancer assessment
International Conference on Toxicology and Pharmacology
November 01-02, 2017 | Toronto, Canada
M Reza Khorramizadeh, Seyed-Behnam Ghaffari, Mohammad-Hossein Sarrafzadeh, Zahra Fakhroueian and Shadab Shahriari
Tehran University of Medical Sciences, Iran University of Tehran, Iran
Scientific Tracks Abstracts : J Clin Exp Tox
Inherent biocompatibility and stability of zinc oxide nanoparticles (ZnO-NPs) and their biomedical potentials make them an emerging candidate for drug delivery. The aim of this study was to develop and assess a simple procedure for surface functionalization of ZnO-NPs by 3-mercaptopropionic acid (MPA) for water-soluble curcumin delivery. Carboxylterminated ZnO nanoparticles were successfully made using ZnCl2 and NaOH in the presence of MPA. The functional groups were activated by 1, 1’-Carbonyldiimidazole (CDI) and the curcumin bonding was carried out at room temperature for 24 h. The core-shell nanocomposite had a significant better solubility versus free curcumin, as characterized by XRD, FTIR, UV-Vis spectrophotometry, DLS, and TEM, p<0.005. In addition, MTT cytotoxicity assessment on MDAMB-231 breast cancer cells revealed a drop of IC50 values from 5 μg/mL to 3.3 μg/mL for free curcumin and ZnOMPA-curcumin complex, respectively. This result showed an augmented cancer-inhibitory effect of nanoconjugate complex. In conclusion, the presented improved solubility and elevated functionality of novel ZnO-MPA-curcumin nanoformula is promising, and could be considered for new therapeutic endeavors.
M Reza Khorramizadeh, is a full Professor at Tehran University of Medical Sciences (TUMS), directs Biosensor Research Center and newly instituted Zebra Fish Core Lab at Endocrinology and Metabolic Molecular-Cellular Sciences Institute. Concurrently, he is a 2nd affiliation to the Dept. of Medical Biotechnology, School of Advanced Technology in Medicine, TUMS.