Journal of Clinical and Experimental Toxicology

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Chemical constituents of donkey dung (Anbarnasara): Questioning the recent claims concerning Therapeutic effects

November 04-05, 2019 | Prague, Czech Republic

Atefeh Hajiagha Bozorgi

Alborz University of Medical Sciences, Iran

Scientific Tracks Abstracts : J Clin Exp Tox

DOI: 10.35841/2630-4570-C2-008


Background: Various animal excrements have been used as a medicine for the treatment of different diseases in the past. Today, people still use some of these excrements, especially female donkey dung (Anbarnasara,) by smelling the smoke of burnt dung for some ailments like sinusitis and bronchitis. Recently, some studies have focused on the therapeutic activities of Anbarnasara but no one has studied the active ingredients of it.

Objective: The aim of this study was to identify the chemical constituents and antibacterial activities of Anbarnasara and its smoke.

Materials and Methods: Female donkey dungs were collected from Shahreza in May 2017. The substance was burnt and the smoke was led to a beaker of methanol by a distillation apparatus. Then, the methanolic extract was dried via a rotary evaporator. Antibacterial activity of the smoke and also the total methanolic extract of Anbarnasara were tested on Staphylococcus aureus and Escherichia coli via well diffusion method. Chemical constituents were analyzed through gas chromatography-mass spectrometry (GC–MS).

Results: The results showed that Anbarnasara and its smoke have a very weak antibacterial activity. Regarding chemical constituents, both total methanolic extract and smoke extract predominantly contained toluene, xylene, and dibutyl phthalate.

Conclusion: Previous studies showed xylene as one of the major components of the smoke of Anbarnasara. These articles also reported the presence of some plant-based compounds such as glyoxal, syringol, and limonene in the smoke. The presence of these compounds is probably due to the donkeys’ diet. In our study, the presence of dibutyl phthalate, an industrial plasticizer, among the components is probably due to environmental factors. These evidences suggested that Anbarnasara is under the influence of environmental factors like vegetation and pollutions and therefore, should be used as a medicine with caution. Recent claims regarding the antibacterial, cytotoxic, and wound healing activities of Anbarnasara should be considered independent of these environmental factors.


Atefeh Hajiagha Bozorgi has completed PharmD from Tabriz university and her PhD from Shahid Beheshti university, Tehran, Iran. She is an assistant professor in Alborz university now and teaches medicinal chemistry courses for pharmacy students. She also manages hospital and educational pharmacies.

E-mail: [email protected]

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