Antiviral activity and possible mechanisms of action of Aristolochia bracteolate against influenza A virus
38th Annual congress on Microbes Infection
September 28-29, 2017 | London, UK
Mona Timan Idriss, Malik SulimanMohamed, Sarawut Khongwichit, Natthida Tongluan, Duncan R Smith, N H Abdurahman and Alamin Ibrahim Elnima
Sudan International University, Sudan
Khartoum university, Sudan
Mahidol University, Bangkok, Thailand
Universiti Malaysia Pahang, Malaysia
Scientific Tracks Abstracts : Microbiology: Current Research
We investigated the anti-influenza virus activity of Aristolochia bracteolate and possible mechanism(s) of action in vitro. We found that Aristolochia bracteolate has anti-influenza-virus activity, and both pre-incubation of virus prior to infection and post-exposure of infected cells with Aristolochia bracteolate extract significantly inhibited virus yields. Influenza-virus-induced hemagglutination of chicken red blood cells was inhibited by Aristolochia bracteolate extract treatment, suggesting that Aristolochia bracteolate can inhibit influenza A virus infection by interacting with the viral hemagglutinin. Furthermore, Aristolochia bracteolate extract significantly affect nuclear transport of viral nucleoprotein (NP). To best of our knowledge, this study revealed for the first time that Aristolochia bracteolate extract can inhibit both viral attachment and replication and offers new insights into its underlying mechanisms of antiviral action. The whole plant of Aristolochia bracteolate collected from Sudan and Extracted with 70% methanol. The crude extract was screened for its cytotoxicity against MDCK cell line by Presto- Blue assay and WST-1 assay. Antiviral properties of the plant extract were determined by cytopathic effect inhibition assay and virus yield reduction assay (plaque assay). Time of addition assay, and nuclear export mechanism were also performed.
Mona Timan Idriss is a Lecturer of Microbiology in the Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Sudan International University, in Sudan. She is currently pursuing PhD studies in Molecular Virology (development of novel antiviral drugs from Sudanese plants and possible mechanism of action). She also worked as a Visiting Scientist in the department of Bimolecular Sciences, University of Mahidol, Thailand. She participated in many projects with members of the Molecular virology laboratory in University of Nagasaki, Japan using molecular biology techniques. Most recently, she has written two papers in virology research. She is selected as an Editorial Board Member for Immunotherapy Research Journal and SciFed Journal of Mycology.