Antagonism of Pseudomonas sp. EMM-1 and its potential as bio-control agent

Antagonism of Pseudomonas sp. EMM-1 and its potential as bio-control agent

Joint Conference on GLOBAL APPLIED MICROBIOLOGY CONFERENCE & International Congress on MICROBIAL & BIOCHEMICAL RESEARCH AND TECHNOLOGIES
October 18-19, 2017 Toronto, Canada

Catherine Cesa-Luna, Joel de la Cruz-Enríquez, Ana Laura Hernandez-Tenorio, Fernando Xicale-Nava, Yolanda Elizabeth Morales-Garcia, Rocio Perez-yTerron, Antonino Baez-Rogelio, Jesus Munoz-Rojas and Veronica Quintero-Hernandez

Universidad Autó noma de Puebla, México

Posters & Accepted Abstracts : Microbiology: Current Research

Abstract:

Statement of the problem: In nature, plants are subject to several diseases due to the presence of pathogens. Fungal diseases are commonly controlled through the use of pesticides which have resulted in clinical and environmental damages. The use of beneficial bacteria may promote diverse beneficial function in intensive agriculture such as biological control, where bacteria can exhibit antagonistic interactions to compete for space and nutrients in their habitat. The best known antagonistic bacteria are Enterococcus, Lactococcus, Streptomyces, Bacillus, Pseudomonas, Klebsiella, Escherichia, and Burkholderia due to their potential to produce inhibitory substances such as broad-spectrum antibiotics, organic acids, siderophores, antifungal and bacteriocins. Our study model, Pseudomonas sp. EMM-1, is a Gram-negative bacterium isolated from contaminated soil highly competitive due to the production of one or more inhibitory substances. Its antimicrobial activity was demonstrated against diverse beneficial and pathogenic microorganisms including the genera Bradyrhizobium, Azotobacter, Staphylococcus, Streptococcus, Klebsiella and Burkholderia; as well as the phytopathogenic fungi Pantoea and Fusarium. Methodology & Theoretical Orientation: Bacterial competition is mainly evaluated by double-layer agar and simultaneous inhibition assays. In this work the double-layer agar methodology was performed to evaluate the ability of Pseudomonas sp. EMM-1 to antagonize diverse fungi isolated from soil and plants with fungal diseases such as Aspergillum and Fusarium. Conclusion & Significance: The results of inhibition assays suggest that Pseudomonas sp. EMM-1 is able to produce metabolites that inhibit the growth of diverse fungi, leading us to assume its potential as biocontrol agent.