A novel immobilized method for isolation of phage display library-derived scFv antibody specific to Listeria monocytogenes
4th International Conference on Medical Microbiology
May 20-21, 2019 | Vienna, Austria
Xuan-Hung Nguyen and Kim-Anh To
Toulouse III University, France Hanoi University of Science and Technology, Vietnam
Posters & Accepted Abstracts : Microbiol Curr Res
We developed a novel simple and cost-effective immobilization method for bio panning of a phagedisplay library. We used Light Expanded Clay Aggregate (LECA) as biomass support matrix to isolate phage display libraryderived scFv antibody specific to Listeria monocytogenes, a bacterium causing serious illness in human and animal. Four rounds of positive selection against LECA-immobilized L. monocytogenes and an additional subtractive panning against L. innocua were performed. This panning scheme in combination with our novel immobilization method allowed us to isolate the phage clones bind to L. monocytogenes without cross-reactivity toward ten other non-L. monocytogenes bacteria. One of the selected phage clones was able to specifically recognize three major pathogenic serotypes (1/2a, 1/2b and 4b) of L. monocytogenes and 11 tested L. monocytogenes strains isolated from foods. This scFv antibody has potential use in development of immunoassay-based methods for rapid detection of L. monocytogenes. In addition, the LECA-immobilization method described here offers an efficient, simple and cost-effective bio panning strategy to isolate specific monoclonal antibodies against any given species of pathogenic bacteria from phage-display libraries.