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Biotechnology Congress 2018 & Emerging Materials 2018
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Volume 29
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Rantumoni Sharma et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C4-010
IN-VITRO
ANTI-OXIDANT AND ANTI-
DIABETIC EVALUATION OF
PREMNA
HERBACEA
ROXB
Rantumoni Sharma, Jayashree Dutta
and
M C Kalita
Rantumoni Sharma, Jayashree Dutta and M C Kalita
P
remna herbacea
Roxb (family-
Verbenaceae
), locally known as
Keradaphini (Bodo) and Matiajam (Assamese), is an undershrub 7-20 cm
high, with sessile leaves and the basal pairs lies flat on the surface. Leaves
of
P herbacea
was collected from Holtugaon reserve forest under Manas
National Park, Assam, India. The plant was authenticated in the GUBH.
The GPS of Holtugaon forest division, from where the plant was collected
is 81´ 13´´N and – 41´ 89´´E. The
in vitro
hypoglycaemic evaluation of the
collected plant material i.e. alpha amylase and alpha glucosidase inhibition
was determined. The anti-oxidant property for their free radical scavenging
property was accessed by DPPH method. The plant material was further
subjected to preliminary phytochemical screening using standard protocol of
Harborne and Trease
et al.
, 1998. The leaf methanol extract of P
herbaceae
demonstrated alpha amylase inhibition with IC50 29.71 μg/mL compared to
acarbose 344.83 μg/mL. While, chloroform extract, petroleum benzene and
ethyl acetate extract demonstrated no inhibition of enzyme alpha amylase. In
case of alpha glucosidase, the methanol extracts demonstrated the highest
inhibition with minimum IC50 value of 382 μg/mL compared to acarbose
397.06 μg/mL followed by the ethyl acetate extract IC50 409.83 μg/mL.
On the other side, chloroform and petroleum benzene demonstrated slight
inhibition with high IC50 value compared to acarbose. The aqueous extract of
Premna herbacea
demonstrated the presence of many bioactive secondary
metabolites like terpenoid, steroid, phenol, flavonoids, saponin, tannin,
coumarin and sugar. However, alkaloid was not detected in
Premna herbacea
.
P herbaceae
demonstrated anti-oxidant property with IC50 value of 4.84 μg/
mL compared to ascorbic acid 5.61 μg/mL. The results obtained showed that
the plant sample is rich with therapeutic properties, which could be a futuristic
alternative for prevailing medicines for anti-oxidant and anti-diabetic.
Rantumoni Sharma has completed his MSc in Botany
(2016) from Gauhati University, India. Currently he is
working in a DBT project under the guidance of Prof
Mohan Chandra Kalita, Department of Biotechnology,
Gauhati University, and he has two publications in re-
puted journals till now including four poster presenta-
tions and one oral presentation.
rantu.rms20@gmail.comBIOGRAPHY