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Biotechnology Congress 2018 & Emerging Materials 2018
Biomedical Research
|
ISSN: 0976-1683
|
Volume 29
S e p t e m b e r 0 6 - 0 7 , 2 0 1 8 | B a n g k o k , T h a i l a n d
allied
academies
Joint Event on
EMERGING MATERIALS AND NANOTECHNOLOGY
BIOTECHNOLOGY
&
Annual Congress on
Global Congress on
Medicharla Venkata Jagannadham, Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C4-010
MASS SPECTROMETRY-BASED
PROTEOMICS: CHALLENGES AND
OPPORTUNITIES
Medicharla Venkata Jagannadham
CSIR-Centre for Cellular and Molecular Biology, India
M
ass spectrometry-based proteomics is extensively used for the
identification of proteins from complex mixtures, their expression,
quantification, post-translational modifications and study the interactions
among proteins. Using proteomics, the biology of several complex diseases
was studied. However, the problems associated with reproducibility, false
discoveries, lack of proper standards, problems in
de novo
sequencing lead
the researcher to look for validation methods. A thorough understanding
of these techniques and their limitations is crucial in facilitating the
development of new strategies to overcome the existing problems and
advancement of the technology. The limitations associated with the
sensitivity, comprehensiveness involved with mass spectrometry should be
understood. Sample preparation, handling and data analysis plays a critical
role on the results. The huge amounts of MS data generated is analyzed
using different algorithms. These algorithms require the protein/genome
data base for the identification of proteins. This approach cannot be used
for the species whose genome sequence is not known. In this situation,
de novo
sequencing followed by homology search is the method of choice
for the identification of proteins. The opportunities in developing different
technologies and strategies in the proteomics work flows play a critical role
in utilizing proteomics effectively.
Medicharla Venkata Jagannadham is working as a
senior Principal Scientist and Project Leader at the
Centre for Cellular and Molecular Biology. He has vast
experience in protein chemistry and proteomics. He
published more than 50 research papers, with some
papers having more than 100 citations. He trained
several students, conductedmeetings and workshops
in proteomics. He received Bharat Jyothi award from
India International Friendship Society, New Delhi in
2014 and Eminent Mass Spectrometrist award from
the Indian Society for Mass spectrometry (ISMAS) in
2015. His current research interests are proteomics,
particularly in improving the
de novo
sequencing ef-
ficiency of peptides using MS techniques, structural
and functional studies of outer membrane vesicles of
Gram-negative bacteria.
jagan@ccmb.res.inBIOGRAPHY