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Biotechnology Congress 2018 & Emerging Materials 2018
Biomedical Research
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ISSN: 0976-1683
|
Volume 29
S e p t e m b e r 0 6 - 0 7 , 2 0 1 8 | B a n g k o k , T h a i l a n d
allied
academies
Joint Event on
EMERGING MATERIALS AND NANOTECHNOLOGY
BIOTECHNOLOGY
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Annual Congress on
Global Congress on
Ashok Kumar Srivastava et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C4-010
CONTINUOUS PRODUCTION OF
BIOPOLYMER (PHB) FROM BY-PRODUCT OF
BIODIESEL INDUSTRY (GLYCEROL) USING
HIGH CELL DENSITY CULTURE
Ashok Kumar Srivastava
and
Kamakshi Gupta
Indian Institute of Technology, India
V
ersatile qualities of strength and lightness of petroleum derived plastics
have an ever-increasing demand in society but are restricted primarily
due to its non-biodegradable nature and its production from disappearing
petrochemical resources. Alternatives are therefore, sought for the polymer
of similar properties which is not only biodegradable but also produced
from renewable resources. Some microbes have shown a distinct ability to
accumulate biodegradable polymer PHB (poly-hydroxybutyrate) when grown
under limiting concentration of nitrogen and phosphates. However, the cost
of production of these polymers is significantly higher than that derived from
fossil fuel resources. Attempts are made to use glycerol (by product of bio-
fuel industry) as substrate to economize the production. To further increase
concentration, yieldandproductivity of bio/copolymer, theprocess isoptimized
to be carried out in fed batch followed by continuous mode with cell retention
or cell recycle device. Batch cultivation of C necator in glycerol exhibited a long
lag phase. To overcome this, attempts were made to use glycerol and glucose
as mixed substrate for above cultivation to improve the process performance.
Statistically optimized concentrations of glycerol and glucose, 25g/l and 5g/l
were used to study growth kinetics in batch mode. The results were used to
develop a mathematical model for the growth of biomass and accumulation
of PHB, which was further extrapolated for fed batch and continuous mode.
To establish a better method for high cell density, both spin filter (cell retention
device) and inclined settler (cell recycle device) were used in separate sets
of experiments. It was observed that spin filter, the cell retention device, has
higher retention efficiency as it produced 12.7g/l biomass and 8.6g/l PHB as
opposed to 10.98g/l biomass and 6.9 g/l PHB in case of inclined settler. The
advantages and limitation of use of either devices for cell retention.
Ashok Kumar Srivastava has received his PhD degree
from the McGill University, Montreal in 1990. He has
40 years of industrial research teaching experience in
Biochemical Engineering and Biotechnology. He has
110 international journal papers, 154 international/
national presentations and two patents to his credit.
He has supervised 16 PhD (five continuing) and 73
master’s theses. His major interest is in modelling
simulation, optimization and control of bioprocesses,
microbial/plant cell/hairy root cultivations for import-
ant metabolite production (bio/copolymer production,
podophyllotoxin, azadirachtin, ajmalicine, shikimic
acid production etc) and novel bioreactor develop-
ment.
ashokks@dbeb.iitd.ac.inBIOGRAPHY