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Biotechnology Congress 2018 & Emerging Materials 2018
Biomedical Research
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ISSN: 0976-1683
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Volume 29
S e p t e m b e r 0 6 - 0 7 , 2 0 1 8 | B a n g k o k , T h a i l a n d
allied
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Joint Event on
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P Anusha et al., Biomed Res 2018, Volume 29 | DOI: 10.4066/biomedicalresearch-C4-010
PRODUCTION OF LOVASTATIN BY SOIL
MICRO FUNGI
RHIZOPUS ORYZAE
P Anusha
and
S Rajkumar Immanuel
The American College, India
N
atural statins like lovastatin which is mainly produced by fungal strains
act as an inhibitor of HMG-CoA reductase, an enzyme involved in the
biosynthesis pathway of cholesterol. The statins decrease the level of
cholesterol in blood. The aim of the study was to determine the lovastatin
producing potential of soil micro fungi. In the present investigation 10 soil
fungi were screened for lovastatin production using shake flask culture.
The screening of potential lovastatin producing fungus was carried out
using bio-assay method against
Saccharomyces cerevisiae
as an indicator
microorganism in the YPDA medium by measuring the zone of inhibition.
The diameter of zone of inhibition ranged from 3.7 to 4.8 mm in
Rhizopus
oryzae
which produced a maximum zone of inhibition. Among the lovastatin
producing strains
Rhizopus oryzae
was found to be utilized maximum
substrates. Genomic identification of the strain was done using 18S rDNA
technique. The DNA of
Rhizopus oryzae
was extracted and purified by agarose
gel electrophoresis and sequenced using the ˜˜1.5kb 18S rRNA fragment
and was amplified using the primers. Phylogenetic analysis performed by the
maximum parsimony (MP) method and molecular evolutionary relationship
was inferred using neighbor–joining method and the identity was confirmed.
The fungi
Rhizopus oryzae
was screened for lovastatin production using
HPLC analysis after extraction of the compound from the fermentation broth
with ethyl acetate. Lovastatin quantification was carried out on extracts from
the culture broth and a production level of 20.39mg/l was recorded. Hence,
from this investigation it was concluded that rapid method of determination
of lovastatin can also be employed to screen lovastatin producing fungal
isolates from soil and the isolate
Rhizopus oryzae
being recommended for
further studies as a potent lovastatin producer.
P Anusha is a pursuing her PhD at The American Col-
lege, India. She is examining her PhD under RajKumar
Immanuel. S RajKumar Immanuel is as of now filling
in as the Associate Professor and Head of the Post-
graduate Department of Environmental Science and
Dean for Curriculum Development and Research at
The American College, Madurai, Tamil Nadu, India. His
zone of research is Fungal Biotechnology.
rajimme102@gmail.comBIOGRAPHY