Microbiology: Current Research

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Commentary - Microbiology: Current Research (2021) Volume 5, Issue 4

Quality assurance in food microbiology and Use of reference materials in quality assurance programmes

Jesse Rotival*

Department of Microbiology, Claude Bernard University, Villeurbanne, France

Corresponding Author:
Jesse Rotival
Department of Microbiology
Claude Bernard University
Villeurbanne
France
E-mail: [email protected]

Accepted date: October 14, 2021

Citation: Rotival J. Quality assurance in food microbiology and Use of reference materials in quality assurance programmes. J Microbiol Curr Res. 2021;5(4):1-2.

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Abstract

Food microbiology is described because the take a look at of the microorganism that inhibit, create, or contaminate meals. This consists of the take a look at of microorganisms inflicting meals spoilage; pathogens which can motive disease (particularly if meals is wrongly cooked or stored); microbes used to supply fermented meals consisting of cheese, yogurt, bread, beer and wine and the beneficial function of microbes in generating probiotics [curd made from lactobacillus].

Description

Quality Assurance (QA) is a way of preventing mistakes and defects in manufactured products and avoiding problems.

Role of quallity assurance

A quality framework as a prerequisite of research center accreditation is making microbiologists survey momentum rehearses. The requirement for Quality Assurance (QA) in food microbial science is of developing significance and this paper presents a clever way to deal with executing QA dependent on a framework which is similar to the Hazard Analysis Critical Control Point approach took on by the food business. The premise of the QA framework is the acknowledgment of Quality Assessment Points (QAPs). A few Quality Control and checking rehearses are proposed for every one of the QAPs with the general point of fostering a Total Quality Assurance framework for food microbial science research facilities [1].

Nine distinctive Reference Materials (RMs) to be used in meals and water microbiology had been advanced with the assist of the European Commission (EC). The manufacturing procedure of RMs is primarily based totally on spray drying microorganism suspended in milk. The exceedingly infected milk powder acquired is blended with sterile milk powder to acquire the preferred stage of infection and is eventually crammed into gelatine capsules. The HCMP can also additionally want to be stabilized through garage for extra than 12 months earlier than a strong RM may be prepared. The HCMP are combined with sterile milk powder the usage of a pestle and mortar to be able to produce homogeneous RMs.

Hazard Analysis Critical Control Point (HACCP) is now nicely hooked up across the world because the method which ought to be applied to make sure manufacturing of secure meal The fundamental standards of this gadget are as compared with an appropriate Quality Assurance necessities in meals microbiology. The key steps are

1. To become aware of Quality Assessment Points (QAPs). These are the factors with inside the manner which want essential evaluation and manipulate.

2. To outline the nice manipulate or tracking these can be visual, physical, chemical or microbiological procedures.

ISO Guide 30 characterizes a CRM as 'a RM at least one of whose property estimations are guaranteed by an in fact substantial technique, Reference Materials (RMs) are characterized by ISO in joined by or discernible to an endorsement or other Guide 30 (Anonymous, 1992) as 'a material or documentation which is given by an affirming body.' substance, at least one properties of which are An illustration of an ensuring body is the Community adequately grounded to be utilized for the Bureau of Reference (BCR) of the European Commission CRMs need to satisfy various materials'. Other than RMs, there are additionally affirmed necessities including

1. Representative for its expected use.

2. Homogeneity indicated with in characterized limits.

3. Stability indicated with limits throughout a predefined timeframe

The RMs investigated consists of period of time. Gelatine capsules containing artificially contaminated spray dried milk, first described in their present form ‘Representative’ means that the RM has to resem-by beckers. The methods of production routine samples RMs have possible to produce a stable and homogeneous RM been developed for use in the field of water and food for every type of food or environmental sample that microbiology. Total of nine different reference materials have been made in order to fulfill the three requirements listed evaluated in collaborative studies involving number of 25 laboratories throughout the European Homogeneity is very important as heterogeneous Union. Based on the results of these studies, a RMs will lead to extra variation in results.

Hetero-number of RMs was subjected to the BCR heterogeneity often exists in natural samples but has to be procedure. In total six RMs have been certified eliminated for RMs as far as possible if the per-using this procedure. The various RMs method or laboratory is to be assessed evaluated in collaborative studies in the field of The distribution of micro-organisms in a homogeneity-water and food microbiology and those certified by sample (for example a fluid) is described by a the BCR. The production process presented Poisson distribution as in this way Highly Contaminated Milk Powders (HCMPs) are produced that can and the normal distribution which can be applied to be used as stock for the production of batches of most other types of analysis (for example chemical) RMs. As a transformation of the counts is needed For microbiological counts a log transformation was used 10 as in most cases this yielded normally distributed data [2,3].

Routine use of RMs

It means that for each series of analysis RMs are examined. A series of analysis is defined as a number of measurements; carried out by one technician in one (part) of a day, using one method, one batch of media etc. The counts obtained from examining the RM can be used for the preparation of a control chart.

Use of certified reference materials

CRMs are used on an occasional basis as the availability of these materials is limited and the price high. CRMs are provided with a certificate and in most cases a certification report. The certificate states the certified values including their 95% confidence limits. These certified values are obtained from a large number of examinations carried out by the participating laboratories during the routine utilization of RMs; implies that for every series of examination RMs are analyzed. A progression of investigation is characterized as various estimations; completed by one professional in one (a piece) of a day, utilizing one technique, one bunch of media and so forth. The counts got from looking at the RM can be utilized for the planning of a control outline.

Utilization of ensured reference materials

CRMs are utilized on an infrequent premise as the accessibility of these materials is restricted and the value high. CRMs are furnished with a declaration and much of the time an accreditation report. The authentication expresses the ensured values including their 95% certainty limits. These ensured values are acquired from countless assessments did by the taking part labs during the confirmation study. They can't utilize straight by a research facility looking at a couple of containers. For use by a certainty limits are given for various blends of cases and duplicates per container. The cutoff points are determined utilizing the guaranteed worth and change parts determined utilizing log changed counts examining only a few capsules. For use by confidence limits are given for different combinations of capsules and replicates per capsule. The limits are calculated using the certified value and variance components calculated using log transformed counts. Transformation of the values obtained for the upper and lower limit will give the limits on the normal scale.

References

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