Case Report - Journal of Clinical Ophthalmology (2022) Volume 6, Issue 3
Intravitreal bevacizumab at the time of phacoemulsification may prevent macular edema in diabetic patients with and without preoperative retinopathy.
Khaled W Elsadi*, Ahmed A Dahab, Iman M Eissa, Adel M Fathy, Khaled E El Rakhawy
Department of Ophthalmology, Cairo University, Giza, Egypt
- Corresponding Author:
- Dr. Khaled W Elsadi
Department of Ophthalmology
E-mail: [email protected]
Received: 03-Sep-2019, Manuscript No. AACOVS-19-2041; Editor assigned: 06-Sep-2019, PreQC No. AACOVS-19-2041(PQ); Reviewed: 20-Sep-2019, QC No. AACOVS-19-2041; Revised: 21-Jul-2022, QI No. AACOVS-19-2041 (QI); Manuscript No. AACOVS-19-2041(R); Published: 18-Aug-2022, DOI: 10.35841/aacovs.6. 2.545-553.
Citation: Elsadi KW, Dahab AA, Eissa IM, et al. Intravitreal bevacizumab at the time of phacoemulsification may prevent macular edema in diabetic patients with and without preoperative retinopathy. J Clin Ophthalmol 2022;6(3):1-6.
Purpose: To evaluate the effect of intravitreal Bevacizumab at the time of phacoemulsification on the incidence of postoperative Diabetic Macular Edema (DME).
Patients and methods: Twenty eyes of diabetic patients with retinopathy yet without maculopathy, and 20 eyes of diabetic patients without retinopathy were included. Only 10 eyes in each group had 1.25 mg Bevacizumab intravitreal injection at the end of surgery. At two weeks, two months and six months postoperative visits, macular optical coherence tomography and visual acuity assessment were done.
Results: At two weeks, two months and six months after surgery, the incidence of DME in the non injected group was 5%, 20%, 30% as opposed to 0%, 5%, 5% in the Bevacizumab group. No statistically significant difference was found in mean central macular thickness. A statistically significant difference in BCVA postoperatively was found between both groups, especially in cases without retinopathy preoperatively.
Conclusion: Prophylactic Intravitreal injection of Bevacizumab at the time of phacoemulsification is effective in the mid-term prevention of DME in cases with and without preoperative diabetic retinopathy.
Bevacizumab, Diabetic macular edema, Phacoemulsification, Non-proliferative diabetic retinopathy.
Diabetic Macular Edema (DME) is the most common cause of visual deterioration in diabetic patients . Recent studies have identified that leakage of fluid and plasma components from the damaged vasculature is mediated by the release of soluble vascular and inflammatory mediators, including Vascular Endothelial Growth Factors (VEGF) and pro-inflammatory cytokines . Studies also show that there is increased level of VEGF in both aqueous and vitreous, and suggest that these levels are correlated with the severity of DME. These evidence suggest that VEGF are an important key in the pathogenesis of DME [3-5].
Cataract surgery is important for improving vision and allowing visualization of the retina for better assessment in diabetic patients. Evidence exists that cataract removal will develop or enhance the progression of diabetic retinopathy and DME even after the implementation of new techniques of cataract surgeries [6,7].
Recent retrospective studies showed there is an increased relative risk of new macular edema development in diabetic eyes after surgery despite the absence of preoperative retinopathy (RR, 1.80; 95% CI, 1.36-2.36). The risk was much higher in the presence of any diabetic retinopathy (RR, 6.23; 95% CI, 5.12-7.58) and rose proportionately with increasing severity of DR . The study highlighted the need for prophylactic therapy whether there is retinopathy or not, especially in the high risk groups .
Based on this relative risk of developing post operative DME even in patients with no diabetic changes preoperatively, the authors searched but did not find enough studies testing the magnitude of progression of DME in these patients, and whether intraoperative injection of Bevacizumab could help decrease this risk. This prospective study was designed to test the possible prophylactic effect of intravitreal Bevacizumab at the time of phacoemulsification on the development of post operative DME in two groups of diabetic patients; a group with a preoperatively normal fundus (A), and a group with preoperative non proliferative retinopathy (B). Both groups had no evidence of macular edema preoperatively. Despite the presence of studies which have tackled post operative DME, only a few of them if any-included diabetic patients with a preoperatively normal fundus. The authors have included this group of patients, along with patients having Non Proliferative Diabetic Retinopathy (NPDR) with no macular edema.
The study protocol was approved by the local review board of the Ophthalmology department at Cairo University, and followed the tenets of declaration. All patients received a thorough explanation of the study aim and procedure done, and all signed a written informed consent.
The study included 40 patients (20 with no diabetic retinopathy, group A) and (20 with NPRD, group B). Patients were consecutively recruited from the outpatient clinic of the retina service unit of Kasr Al-Ainy hospital. Within each group (A and B), patients were further allocated (by simple randomization computer software) to two subgroups; a Bevacizumab group (A/Bzb and B/Bzb) who received injections at the end of phacoemulsification, and a control group (A/Ctrl and B/Ctrl) who did not receive injections at the end of surgery. Initially, slightly more than 20 patients were consecutively recruited for each group, to allow for possible follow up drop outs or patients with surgical complications to be excluded. We eventually included 20 patients in each group.
All included patients had diabetes for ± 11 years and had significant nuclear cataract or dense posterior subcapsular cataract. The posterior segment was assessed by slit-lamp biomicroscopy and by macular OCT (RTVue Fourier-Domain OCT, v 126.96.36.199, Optovue Inc., USA) to confirm the absence of DME preoperatively. The Best Corrected Visual Acuity (BCVA) was measured before and after surgery using a Snellen’s chart for all patients and converted the reading to logMAR.
All cases underwent surgery when all systemic conditions were controlled (blood sugar and blood pressure). A standard phacoemulsification procedure was done and a foldable hydrophilic acrylic intraocular lens (of the same brand) was implanted in all patients. However, in the Bevacizumab injected (A/Bzb, B/Bzb) group of patients, at the end of surgery intravitreal 1.25 mg Bevacizumab injection (Avastin®, Genentech Inc., San Francisco, CA) was given through 30- gauge needle, introduced 3.5 mm from the limbus supratemporally under the surgical microscope.
Postoperative follow up examinations were scheduled, on the first day and at two weeks, two months and six months postoperatively. All subjects completed their follow up after surgery. All patients were subjected to fundus examination by slit-lamp biomicroscopy and macular mapping by optical coherence tomography (RTVue Fourier-Domain OCT, 188.8.131.52, Optovue Inc., USA) postoperatively.
Statistical analysis was carried out using the statistical package SPSS (Statistical Package for the Social Science; SPSS Inc. Chicago, IL, USA) version 22. Wilcoxon Signed Ranks test was used for data analysis within the groups and Mann- Whitney test for analysis between the Bevacizumab and control subgroups. Chi-squared test was used for the qualitative data. All tests were two-tailed and considered significant at p ≤ 0.05 and highly significant at p<0.001.
Forty eyes of 40 diabetic patients with no DME (as confirmed by OCT) were studied. Twelve of the recruited patients were males (30%) and 28 were females (70%). The mean descriptive statistics for both main groups are shown in Table 1.
|Control (A+B)||Bevacizumab (A+B)|
|Age (mean ± SD)||60.8 ± 6.9||60.5 ± 6.4|
|Duration of diabetes in years (mean ± SD)||11.4 ± 3.9||11.7 ± 5.3|
|Insulin (%)||8 (40)||8 (40)|
|OAD (%)||12 (60)||12 (60)|
|Hypertensive (%)||6 (30)||11 (55)|
Note: SD: Standard Deviation, OAD: Oral Anti-Diabetic
Table 1. Descriptive statistics of diabetic patients in the 2 main groups.
It is worth mentioning that in comparisons, the authors will at times compare all injected (A/Bzb+B/Bzb) to all non injected (A/Ctrl+B/Ctrl) subjects (regardless of their retinopathy status) and will sometimes compare the normal fundus subjects (group A) to the NPDR subjects (group B).
Firstly, upon comparing all injected individuals in the two main groups (A/Bzb+B/Bzb) to all control ones in both groups (A/ Ctrl+B/Ctrl) the authors found that:
Collectively, the incidence of DME at 2 months post operatively was 4 eyes (20%) in all control subjects altogether, versus only one eye (5%) in all injected subjects altogether. At 6 months postoperatively the incidence of developing DME was 6 eyes (30%) within all control subjects and one eye (5%) within all injected subjects (p-value=0.04) (Table 2). The difference at 6 months was statistically significant.
|Control (A+B)||Bevacizumab (A+B)||P* value|
|DME at 2 weeks (%)||1 (5%)||0 (0%)||N/A|
|DME at 2 months (%)||4 (20%)||1 (5%)||0.15|
|DME at 6 months (%)||6 (30%)||1 (5%)||0.04|
Note: * Chi-Squared test (p value<0.05 is considered statistically significant), DME: Diabetic Macular Edema. N/A: Not Applicable
Table 2. Collective incidence of postoperative DME in all (controls and Bevacizumab injected) subjects.
We also compared the Central Macular Thickness (CMT) in microns between both types of subjects (all injected subjects versus all controls) postoperatively by OCT. Table 3 shows there was no statistically significant difference between these two collective groups in the mean CMT at 2nd week, 2nd months and 6th months after surgery (p>0.05).
|Control (A+B)||Bevacizumab (A+B)||P* value|
|(mean ± SD)||(mean ± SD)|
|Baseline (μm)||229.4 ± 26.8||230.8 ± 26.7|
|2nd week (μm)||254.3 ± 34.3||238.9 ± 19.6||0.78|
|2nd month (μm)||274.4 ± 60.8||250.6 ± 23||0.86|
|6th month (μm)||278.3 ± 52.3||254.7 ± 26.2||0.11|
Note: *Mann-Whitney test (p-value<0.05 is considered statistically significant), CMT: Central-Macular Thickness, SD: Standard Deviation
Table 3. CMT at baseline compared to 2 weeks, 2 months and 6 months after phacoemulsification in all injected versus all control subjects.
However, a statistically significant difference in CMT was found when comparing baseline CMT to the last follow-up visit between both groups, with higher increase in CMT lying in (all) control subjects (p?0.001) versus (all) injected ones (p>0.02) denoting a potential protective role for Bevacizumab against DME (Table 4).
|Group||Baseline (μm)||6 months (μm)||P* value|
|(mean ± SD)||(mean ± SD)|
|Control (A+B)||229.4 ± 26.8||278.3 ± 52.3||<0.001|
|Bevacizumab (A+B)||230.8 ± 26.7||254.7 ± 26.2||0.002|
|A/Ctrl||230.4 ± 23.2||272.8 ± 36.2||0.007|
|B/Ctrl||228.4 ± 31.2||283.8 ± 66.3||0.005|
|A/Bzb||236.8 ± 28.5||254.9 ± 30.9||0.074|
|B/Bzb||224.7 ± 24.8||252.5 ± 21.8||0.012|
Note: * Wilcoxon Signed Ranks test (p value<0.05 is considered statistically significant); A/Ctrl: Normal fundus controls; B/Ctrl: NPDR fundus controls; A/Bzb: Normal fundus injected with Bevacizumab; B/Bzb: NPDR fundus injected with Bevacizumab; SD: Standard Deviation; CMT: Central Macular Thickness
Table 4. Comparison between CMT at baseline and at 6 months follow-up in all subgroups.
The BCVA was assessed by Snellen chart and then converted to LogMAR reading. The mean BCVA was improved in controls (A+B) as well as Bevacizumab (A+B) subjects of both groups, with higher achieved BCVA in injected subjects (A/Bzb+B/Bzb) than non-injected ones (A/Ctrl+B/Ctrl) at 2 weeks (p<0.012), 2 months (p<0.007) and 6 months (p<0.008) respectively. Table 5 describes the results of comparing baseline BCVA with that of the last follow-up visit in all six subgroups (injected versus controls, as well as normal fundus subgroups versus NPDR subgroups).
|Group||Baseline (LogMAR)||6 months (LogMAR)||P* value|
|(mean ± SD)||(mean ± SD)|
|Control (A+B)||0.79 ± 0.24||0.28 ± 0.09||<0.001|
|Bevacizumab (A+B)||0.69 ± 0.26||0.19 ± 0.07||<0.001|
|A/Ctrl||0.78 ± 0.23||0.28 ± 0.07||0.005|
|B/Ctrl||0.82 ± 0.27||0.28 ± 0.11||0.005|
|A/Bzb||0.62 ± 0.19||0.19 ± 0.08||0.005|
|B/Bzb||0.77 ± 0.30||0.19 ± 0.07||0.005|
Note: * Wilcoxon Signed Ranks test (p value< 0.05 is considered statistically significant). A/Ctrl: Normal fundus controls; B/Ctrl: NPDR fundus controls; A/Bzb: Normal fundus injected with Bevacizumab; B/Bzb: NPDR fundus injected with Bevacizumab; SD: Standard Deviation; BCVA: Best Corrected Visual Acuity.
Table 5. Comparison between CMT at baseline and at 6 months follow-up in all subgroups.
BCVA significantly improved over baseline at 6 months follow up in all subgroups (p?0.001, p 0.005) respectively.
Further statistical analysis was done to highlight the difference between group A (previously normal fundus) and group B (NPDR fundus). This was done to assess if there was any statistical difference between a preoperative pathological retina and a morphologically free retina.
The difference in CMT was not found to be statistically significant in each follow-up visit when comparing group A to group B controls, or group A to group B injected subjects (Table 6).
|Group A/Ctrl||Group B/Ctrl||P* value||Group A/Bzb||Group B/Bzb||P* value|
|(mean ± SD)||(mean ± SD)||(mean ± SD)||(mean ± SD)|
|Baseline (μm)||230.4 ± 23.2||236.8 ± 28.5||228.4 ± 31.2||224.7 ± 24.8|
|2nd week (μm)||250.9 ± 27.8||242.2 ± 17.5||0.44||257.6 ± 41.1||235.6 ± 21.9||0.11|
|2nd month (μm)||266.3 ± 31.6||254.7 ± 27.2||0.53||282.5 ± 81.7||246.4 ± 18.5||0.11|
|6th month (μm)||272.8 ± 36.2||254.9 ± 30.9||0.44||283.8 ± 66.3||252.5 ± 21.8||0.19|
Note: * Mann-Whitney test (p value< 0.05 is considered statistically significant). A/Ctrl: Normal fundus controls. B/Ctrl: NPDR fundus controls. A/Bzb: Normal fundus injected with Bevacizumab; B/Bzb: NPDR fundus injected with Bevacizumab; SD: Standard Deviation; CMT: Central-Macular Thickness.
Table 6. Comparison between CMT at baseline and at 6 months follow-up in all subgroups.
While comparing CMT between preoperative and last followup visit, there was a statistically significant difference in thickness in all subgroups (denoting progression of DME) except subjects of the normal fundus group who received Bevacizumab; A/Bzb (p<0.074).
This statistical finding highlights that the least progression in macular thickness postoperatively was achieved in the subgroup with a normal fundus preoperatively who also received prophylactic intraoperative Bevacizumab (A/Bzb).
The same analysis was also done for BCVA, and revealed a statistically significant higher improvement in BCVA in normal fundus subjects who received Bevacizumab (A/Bzb) than controls within the same group (A/Ctrl) at all stages of follow up (p<0.05). Meanwhile, there was no statistically significant difference in BCVA between injected (B/Bzb) and non-injected subjects (B/Ctrl) in the NPDR group (p value>0.05) (Table 7).
|BCVA||Group A/Ctrl||Group A/Bzb||P* value||Group B/Ctrl||Group B/Bzb||P* value|
|(LogMAR)||(mean ± SD)||(mean ± SD)||(mean ± SD)||(mean ± SD)|
|Preoperative||0.78 ± 0.23||0.62 ± 0.19||0.82 ± 0.27||0.77 ± 0.30|
|1st day||0.47 ± 0.15||0.29 ± 0.13||0.019||0.43 ± 0.17||0.33 ± 0.15||0.143|
|2nd week||0.38 ± 0.11||0.25 ± 0.09||0.029||0.36 ± 0.15||0.26 ± 0.10||0.218|
|2nd month||0.29 ± 0.08||0.19 ± 0.08||0.035||0.28 ± 0.11||0.19 ± 0.07||0.123|
|6th month||0.28 ± 0.07||0.19 ± 0.08||0.043||0.28 ± 0.11||0.19 ± 0.07||0.123|
Note: * Mann-Whitney test (p value<0.05 is considered significant). A/Ctrl: Normal fundus controls; A/Bzb: Normal fundus injected with Bevacizumab; B/Ctrl: NPDR fundus controls; B/Bzb: NPDR fundus injected with Bevacizumab; SD: Standard Deviation; BCVA: Best Corrected Visual Acuity
Table 7. Comparison between CMT at baseline and at 6 months follow-up in all subgroups.
While comparing BCVA at baseline to the last follow-up in all subgroups, revealed a statistically highly significant difference (p<0.005) with improvement noted in all 4 subgroups.
Finally, there was no development of retinopathy in any of the normal fundus patients (group A), nor there was progression of retinopathy in the NPDR group (group B) during the six months follow up.
Cataract surgery is evolving to be a safer procedure with shorter recovery time and higher visual outcome. However, DME is still a major challenge as it commonly follows uneventful phacoemulsification in diabetics . DME results from biochemical and cellular changes such as increased VEGF, increased retinal vascular permeability and impaired blood retinal barrier which lead to leakage and exudation [8-10].
A ten time increase in the level of VEGF is found in the aqueous of diabetic patients versus non-diabetic patients first day postoperatively . Bevacizumab is a commonly used anti-VEGF in DME. Studies claimed that intravitreal Bevacizumab in earlier DME stages will give better visual acuity outcomes .
Our study found that the incidence of DME was 30% in controls of both groups (A/Ctrl+B/Ctrl) and 5% in all Bevacizumab injected subjects of both groups (A/Bzb+B/Bzb) (p value 0.04) which is comparable with the results of a study done where the incidence of DME was 33% in the control group and 3% in the bevacizumab group. Their study examined the effect of intravitreal Bevacizumab combined with phacoemulsification in diabetic patients with a follow up of two months. Our study offered a longer follow up period, as well as further subgrouping of patients. Our study found a statistically significant difference between the main groups in BCVA (p>0.007), which is comparable to their study (p value>0.005) .
On the other hand, a study on 54 patients divided into control and Ranibizumab groups with a follow up of three months. They recorded an incidence of 26% in the control group and 4% in the Ranibizumab group which is comparable with our results despite using a different type of anti-VEGF agent .
In our study, the incidence of DME in patients in group B (NPDR group) was 30%. This incidence is higher found (22%). They assessed the incidence of DME in 50 diabetic patients (some with no retinopathy, some with NPDR and some with PDR) after cataract surgery and followed them up for 3 months. We postulate that we reported higher incidence probably due to our longer follow up period .
Our study found no statistically significant difference between the two main study groups and subgroups in central macular thickness over the follow up period. Their study (61 patients with NPDR with no DME and divided into a control and a Bevacizumab groups), which followed patients up for 6 months, found no statistically significant differences between the two studied groups in central macular thickness at six months . They also reported no statistically significant difference between the two study groups in BCVA at six months posoperatively, a finding which did not agree with our results, as we found a statistically significant improvement in BCVA especially in patients with a preoperatively normal fundus.
Our study highlighted the better visual outcome as well as better baseline to follow up progression of macular edema in a newly studied subgroup of patients; the diabetic patients without retinopathy. Whether routine intravitreal Bevacizumab should be given to diabetic patients-regardless of the presence or absence of retinopathy at the time of phacoemulsification is our proposed recommendation. This may however, need further studies from other authors at different areas of the world to confirm that our results are reproducible.
There are limitations to our study. First, the relatively small number of patients in each subgroup, which despite shedding light on the fact that patients with no diabetic changes before surgery were the subgroup with best prognosis postoperatively, still need to be further confirmed with larger patient numbers in further studies. Second, the six month follow up period,which despite being longer than most other studies, could be prolonged further to one year or more after surgery.
In conclusion, the incidence of DME is high after phacoemulsification. The use of intravitreal Bevacizumab at time of phacoemulsification could offer a safe and effective prophylactic modality in the short-term prevention of postoperative DME for cases with and without preoperative retinopathy.
Declaration of Interest
The authors report no financial interest to any of the material or instruments used in this study.
No funding or grants were received for this study.
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