Journal of Nutrition and Human Health

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Mini Review - Journal of Nutrition and Human Health (2022) Volume 6, Issue 4

Among a mouse cancer paradigm, comparative effects of long-term intermittent calorie reduction versus chronic calorie restriction on peroxidation.

Shu Nuo*

Department of General Medicine, Minhang Hospital, Fudan University, 201199 Shanghai, China

*Corresponding Author:
Shu Nuo
Department of General Medicine
Minhang Hospital, Fudan University
201199 Shanghai, China
E-mail: [email protected]

Received: 01-Apr-2022, Manuscript No. AAJNHH-22-119; Editor assigned: 04-Apr-2022, Pre QC No. AAJNHH-22-119(PQ); Reviewed: 18-Apr-2022, QC No. AAJNHH-22-119; Revised: 21-Apr-2022, Manuscript No. AAJNHH-22-119(R); Published: 28-Apr-2022, DOI: 10.35841/aajnhh-6.4.119

Citation: Nuo S. Among a mouse cancer paradigm, comparative effects of long-term intermittent calorie reduction versus chronic calorie restriction on peroxidation. J Nutr Hum Health. 2022;6(4):119

Visit for more related articles at Journal of Nutrition and Human Health


Cancer is the second leading cause of death within world, accounting for 24% of all causalities per year. Breast cancer is the most commonly diagnosed malignancy in postmenopausal women of all cancer kinds. Melanoma, on the other hand, remains incurable. As a result, more efficient and effective ways for prognosis tactics, treating, and, most importantly, safety are required. Breast cancer includes various risk factors, including inherent factors like age, ethnicity, race, and history, as well as extrinsic factors like smoking, excessive alcohol consumption, lack of exercise, a poor diet, and overweight [1]. Whereas oestrogen, Incretin Growth Factor (IGF), Mammalian Target of Rapamycin (mTOR), leptin, and adiponectin signal transduction were proposed for each relationship between obesity and breast cancer development, the actual molecular mechanism of this interaction is still to be established. Among the most successful weight treatments is calorie restriction. Furthermore, in preclinical malignant tumors, it has been shown to extend longevity and prevent a range of illnesses, including cancer. Physiological changes as a result of calorie restriction enhance survivability traits such as increased insulin tolerance and lower blood glucose, insulin like growth signalling, inflammation, and angiogenesis. During mammary tumour prevention trials, there were three general types of CR regimens used. Chronic calorie restriction and intermittent calorie restriction are the two methods. When compared to CCR, the latter can be demonstrated to be more effective at reducing tumour frequency and greatly delaying tumour growth delays [2].


Female MMTV-TGF- (C57BL/6) mice were used. In the second half of their lives, MMTV-TGF- mice develop the disease, which has some similarities to human breast cancer development. Living beings, a component of the epidermal growth factor receptor EGFR and ErbB cascade that plays an important role in cancer development, is overexpressed in all these mice. Mice received unrestricted access to piped water and kept in small cages in a chamber with a temperature of 21- 24°C and a 12-hour light/dark cycle. Every day, the creatures are checked for every signs of illness [3].

Concept of an experimental

Around 1 month of age, MMTV-TGF- (C57BL6) female mice were randomly assigned to three dietary groups: AL, CCR, or ICR. Altromin TPF1414 meals were acquired from Kobay Ankara, Turkey, and fed to every animals [4]. Everywhere in investigation, mice in the AL group had unlimited access to food. Mice in the CCR group were given 85% food consumption of their age-matched Cetera counterparts, resulting in a 15% calorie reduction when compared to the AL group. Rodents in the ICR group were fed of Cetera mice's calorie intake for one week before being fed AL for the next three weeks, resulting in a 15% calorie reduction when contrasted to AL mice. ICR mice were subjected to this cyclical regimen until they were sacrificed at certain time intervals. Each day at 9:00 am, all rats were fed and their food was recorded. Every Monday at 9 am, body weight gain were taken. A veterinary assessed the animals’ health on a regular basis. Mice were rats were fasted and blood samples were taken by retro orbital haemorrhage at 9 am. After which the mice were sacrificed into a vacutainer tube containing an anticoagulant. A specified ages were four months baseline, 17,18 weeks, 49,50 weeks, and 81,82 weeks. The ICR-fed mice were separated into two parts. Transfected animals were those from which blood samples were taken at the end of three weeks of AL feeding weeks. ICR-restricted studies were those in which blood samples were taken at the end of the one-week CR phase weeks. Previous research indicates no variations in numerous metrics, particularly body weights, between the AL and CCR groups for 1-week changes. As a result, the data for AL and CCR groups at each time point were pooled [5].

Erythropoiesis sample preparation

Upper erythrocytes were cleaned three times with isotonic saline solution after blood samples were centrifuged and plasma was obtained from the top of the test tube. All cell debris was again separated by centrifugation after a specified amount of erythrocytes were lysed with cold distilled water. These supernatants were transferred to clean tubes until the experiments are completed. Another Lowry technique to determine the protein content of a leukocyte solution.

Detecting lipid peroxidation

The amounts of lipid oxidation in erythrocytes were measured using the enhanced Placer method. The approach is based on the pink hue produced by the reaction of MDA with thiobarbituric acid TBA, Sigma Aldrich, T5500. Trichloroacetic acid TCA, Sigma Aldrich, 27242 and phosphate - buffered mixture were applied to samples and left at 4°C for 2 hours. During incubation, samples were centrifuged for 15 minutes at 4400 speed. The supernatant was then extracted, and also the supernatant was vortexed using deionized ethylene diamine tetra acetic acid EDTA, Sigma Aldrich, E5134 and TBA. Thermo Scientific Evolution 300 spectrometer measured absorption at 532 nm after 15 minutes in a water bath [6].


The positive effects of lifespan and fitness are the result of extreme dieting and slowed metabolism, which reduce Production of reactive oxygen species and avoid cell damage. MDA is a type of lipid peroxidation product that is produced when radicals assault lipid. It plays various hazardous and mutagenic roles, and its capacity to attach to molecules allows it to influence multiple signalling pathways. They further suggest that oxidative stress may be one of the pathways linking Change request to the development of breast cancer. Understanding the mechanisms of CR's cancer-protective properties in detail is critical for developing more effective drugs, treatments, and prevention techniques.


  1. Siegel RL, Miller KD, Jemal A. Cancer statistics, 2016. CA Cancer J Clin. 2016;66(1):7-30.
  2. Indexed at, Google Scholar, Cross Ref

  3. Ferlay J, Soerjomataram I, Dikshit R, et al. Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN 2012. Int J Cancer. 2015;136(5):359-86.
  4. Indexed at, Google Scholar, Cross Ref

  5. Gnerlich JL, Deshpande AD, Jeffe DB, et al. Poorer survival outcomes for male breast cancer compared with female breast cancer may be attributable to in-stage migration. Ann Surg Oncol. 2011;18(7):1837-44.
  6. Indexed at, Google Scholar, Cross Ref

  7. Macacu A, Autier P, Boniol M, et al. Active and passive smoking and risk of breast cancer: A meta-analysis. Breast Cancer Res Treat. 2015;154(2):213-24.
  8. Indexed at, Google Scholar, Cross Ref

  9. Park SY, Kolonel LN, Lim U, et al. Alcohol consumption and breast cancer risk among women from five ethnic groups with light to moderate intakes: The multiethnic cohort study. Int J Cancer. 2014;134(6):1504-10.
  10. Indexed at, Google Scholar, Cross Ref

  11. James FR, Wootton S, Jackson A, et al. Obesity in breast cancer-What is the risk factor?. Eur J Cancer. 2015;51(6):705-20.
  12. Indexed at, Google Scholar, Cross Ref

Get the App