Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +1 504 6082390

Research Article - Biomedical Research (2017) Volume 28, Issue 7

The effect of nerve block alone or with zinc on wound healing in a rat model of diabetic foot ulcer

Introduction: Patients with Diabetic Foot Ulcer (DFU) have a high risk of limb amputation as well as higher five-year mortality rates than those for several types of cancer. Effective treatment of DFU, therefore, is a pressing issue. Nerve block can have a beneficial wound-healing effect in this regard, due to its sympathectomy-like vasodilation promoting blood circulation. Zinc furthermore, as a cofactor, can facilitate wound healing. In this study, we evaluated the wound-healing effects of nerve block alone and with Intravenous (IV) zinc.

Materials and Methods: Fifteen (15) male Otsuka Long-Evans Tokushima Fatty (OLETF) rats were equally divided into three DFU groups: a non-treated (group 2), a nerve-block (group 3), and a nerveblock and IV zinc group (group 4). Five additional male Long-Evans Tokushima Otsuka (LETO) rats were assigned to a control, non-diabetic group (group 1). A full-thickness wound (5 mm × 5 mm) was made in all of the rats’ left dorsal foot, which, on post-injury day 1, was treated with sciatic nerve block alone (group 3) or additionally with IV zinc (group 4). On day 13, the wound size was measured, and a histological examination was performed with haematoxylin and eosin (H & E) and Masson’s trichrome staining.

Results: The wound-surface-area differences among the three DFU groups were not statistically significant (p=0.40). Neither were there any significant histological differences.

Conclusion: In this study, neither intermittent nerve-block alone or with IV zinc affected DFU healing.

Author(s): Yusom Shin, Tae Woo Park, Joo Won Kim, Min Jung Jung, Hee Bin Park

Abstract Full Text PDF