Research Article - Biomedical Research (2018) Volume 29, Issue 12
In vitro antitumor activity of chemical constituents of EtOAc extract from Artemisa gmelinii
Objective: To investigate the chemical composition of EtOAc extract from Artemisa gmelinii Web. ex Stechm. (A. gmelinii) and evaluate their in vitro antitumor activity.
Methods: The EtOAc crude extract from the aerial parts of A. gmelinii was isolated by chromatography and the structures of the isolated compounds were elucidated based on spectral analysis. All the isolated compounds were investigated for their in vitro activity against four human cancer cell lines by SRB (Sulforhodamine B) assay.
Main Findings: Eight compounds, namely ameliaroside (1), annphenone (2), 6, 8-dimethoxycoumarin-7- O-β-D-glucuronide (3) 6-methoxycoumarin-7-O-β-D-glucuronide (4), sacroflavone A (5), sacroflavone B (6), sacric acid A (7) and sacric acid B (8) were isolated from the EtOAc extract from A. gmelinii. Compounds 3-6 have certain activity against these tested human cancer cell lines. Among of them, compound 5 (IC50: 5.03-6.78 μmol/L) was found more potent than those of the reference Etoposide (IC50: >50 μmol/L) against Hela and MKN-45.
Conclusion: Compounds 1-8 are isolated from this plant for the first time. Compound 5 (IC50: 5.03-6.78μmol/L) was found more potent than those of the reference Etoposide (IC50: >50 μmol/L) against Hela and MKN-45. Compound 5 have good antitumor effect which may be used as potential antitumor agent.Author(s): Yu Hua, Qinghu Wang, Biligetu Pa, Wenqiang Bao, Junsheng Hao, Jingjing Han