Research Article - Journal of Biochemistry and Biotechnology (2022) Volume 5, Issue 5
Effect of Xylaria nigripes, Panax notoginseng, Cuscuta chinensis on GABA, GABA transaminase, glutamate decarboxylase, amyloid beta, and amyloid beta aggregation levels in PC12 neuron cells.
The purpose of this study is to determine the effect of three different botanical compositions, Xylaira Nigripes extract (XE), Xylaira Nigripes + Panax notoginseng extract (XPE), and Xylaira Nigripes+Panax notoginseng+Cuscuta chinensis extract (XPCE) on γ-Aminobutyric acid (GABA) content, GABA transaminase (GABA-T), Glutamate decarboxylase (GAD), cells viability, and Amyloid-β protein (Aβ) aggregation levels in PC12 neuron cell. For this study, different concentrations (40, 60, 80, and 120 μg/mL) of the three compositions were used and all the experiments were performed in triplicate. All three compositions could significantly improve (p<0.001) GABA production and also increase the activity of glutamate GAD along with decreased GABA-T activity in PC12 cells in a dose-dependent fashion. Moreover, all three compositions showed potent neuroprotective properties via increasing PC12 cell viability as well as significantly attenuating (p<0.001) the Aβ-induced injury and Aβ aggregation in a dose-dependent manner. Overall, all the three compositions exhibited strong neuroprotective activity by improving GABA release through elevated GAD activity with decreased GABA-T activity. Also, all three compositions enhance the proliferation (cell viability) of PC 12 cells and significantly lowers the Aβ aggregation. Out of the three compositions, XPCE (p<0.001) showed the best holistic neuroprotective and GABA releasing properties than XE and XPE.
Author(s): Su-Fen Huang1, Han-Wen Yang1, Kamesh Venkatakrishnan2, Tsu-Chung Chang1*, Wen-Liang Chang3*