Perspective - Journal of Genetics and Molecular Biology (2021) Volume 5, Issue 5
Design and Use of a Flexible RNAi Expression Vector in Mammalian Cells .
We present pRIGHT11, a flexible mammalian expression vector
for the generation of short interfering RNA (siRNA) in cells.
To stimulate the expression of short siRNA, this vector uses
opposing eukaryotic RNA polymerase III promoters U6 and
H1. We've shown that pRIGHT11-derived siRNA can inhibit
transfected reporter genes and endogenous genes in a sequencespecific
manner. This retrovirus-based vector can also carry a
random library of siRNA, allowing for quick identification of
novel genes involved in specific cellular responses. Author(s): Fleur Delacour