Biomedical Research

All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Reach Us +1-504-608-2390

- Biomedical Research (2014) Volume 25, Issue 3

An experimental Study on the mechanism for IL-8 promoting migration of A549 cells.

The effect and mechanism of IL-8 on A549 cells migration were investigated. The appropri-ate concentration of IL-8 was chosen by MTT assay. The Western-blot assay, Scratch test and Transwell assay were used in this study to analyse the effect and mechanism of IL-8 on lung adenocarcinoma A549 cells migration. The results of Western-blot assay showed that: (1) IL-8 can promote the expression of MMP-2 protein and has no effect on the expression of MMP-9. (2) IL-8 can promote the expression of JNK/SAPK phosphorylated protein. (3) In-hibitor (SP600125) can block the effect of IL-8 on the expression of MMP-2 protein. Scratch test proved from the opposite side that the low expression of MMP-2 could inhibit the mi-gration of A549 cells. These results suggest that IL-8 can regulate MMP-2 protein expression through the JNK/SAPK signaling pathway and further promote the migration of lung ade-nocarcinoma A549 cells.

Author(s): Jiang Yuequan, Cai Huarong, Zhou Hong, Zhang Zhi

Abstract Full Text PDF

Get the App

https://pmasites.com