This study aimed to determine the nutritional value, chemical composition and anti-obesity effect of dried Jojoba leaves, and to evaluate its histopathology and hematology parameters effects in rats fed diet provided the aqueous extract of Jojoba leaves. Jojoba leaves were dried by shadow, sun, and oven at 100°C for 30, 60 and 90 min and the chemical composition was determined. The oven dried at 100°C for 30 min (OD1 treatments) have been chosen to evaluate its effect on rat’s body as the balanced drying treatments. 1%, 2% and 3% concentrates of OD1 Jojoba leave aqueous extract evaluate its effect on fat, protein and water content of rats feed on them for 12 weeks. Body weight, histopathology and hematology parameters were analyzed. The results indicated that the aqueous extract of OD1 Jojoba is rich in protein, phenolic, tannins compounds, and low percent of simmondsin. Animals fed diet with 3% (AEJL) showed decreased 24% of body weight. Non-significant effects on hematology parameters and no remarkable histopathologic changes were noted in the liver and kidney weights. in our study, aqueous Jojoba leaves extract with low percent amount of simmondsin, at both the 1%, 2% and 3% concentrations were significantly reduced body weight without significant negative effects.