Asian Journal of Biomedical and Pharmaceutical Sciences

Asian Journal of Biomedical and Pharmaceutical Sciences 44 7897 074717

Ion Exchange Chromatography Scholarly Peer-review Journal

Ion chromatography (or ion-exchange chromatography) is a chromatography process that separates ions and polar molecules supported their affinity to the ion exchanger. It works on almost any quite charged molecule—including large proteins, amino acids, and small nucleotides. However, ion chromatography must be wiped out conditions that are one unit far away from the isoelectric point of a protein.

The two sorts of ion chromatography are anion-exchange and cation-exchange. Cation-exchange chromatography is employed when the molecule of interest is charged. Anion-exchange chromatography is used when the molecule of interest is negatively charged.

One of the first advantages for the utilization of ion chromatography is merely one interaction involved during the separation as against other separation techniques; therefore, ion chromatography may have higher matrix tolerance. Another advantage of ion exchange, is the predictability of elution patterns (based on the presence of the ionizable group). For example, when cation exchange chromatography is employed , cations will elute out last. Meanwhile, the negative charged molecules will elute out first.

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