+44-7897-074717Whole blood automation: Technology for blood components preparation in Blood
3rd International Conference on Health Care and Health Management
November 04-05, 2019 | Prague, Czech Republic
Haw Boon Hong
Khon Kaen University, Thailand
Posters & Accepted Abstracts : J Public Health Policy Plann
Abstract:
Background and objective: The Reveos automated blood
processing system has been developed for whole blood units
separation. The aim of this study was evaluate the product
specifications and quality of components produced by the
Reveos system. This system has been installed in Blood
Transfusion Centre, Faculty of Medicine, Khon Kaen University
since September 2016 for leukocyte reduction (LR) set and in
2019, Non-leukocyte reduction (NLR) set was developed for
the same machine, therefore LR and NLR set were consider to
routine blood collection, then the validation have to perform.
Materials and Methods: The Reveos NLR set-4NG456S0
can separate blood components; Leuko-poor RBCs, Plasma
and platelets during the procedure by centrifugation and
buffy coat removal by automation system. Whole blood
(WB) volume including CPD based on WB collection volume
450+10% mL. And centrifugation by selective protocol of
Reveos. WB was processed using the Reveos system and
compare according to validate the use of the first installation.
Reveos red cells were leukoreduced and stored in SAGM
at 4°C. Reveos plasma was frozen at -30°C and factor
activity was assessed after thawing. Leukoreduced platelet
concentrates Reveos were prepared by pooling 4 iso-group
interim platelet units or 1-2 random platelet concentrate
from top-bottom method and 3-2 interim platelet units
pooled by Reveos pooling set, Comparison the value of
volume, hematocrit , platelet contents and white blood cell
contamination of Leukocyte depleted red blood cells (LDPRC)
and Leukocyte poor red blood cells (LPRC) with SPSS statistics.
Results: 408 whole blood was processing with Reveos
system. Average of fresh frozen plasma, interim platelet and
leuko-packed is 217.52, 62.40 and 10.2 mL., respectively. The
platelet index more than 60 cells/u is 75.8%. The quality of
LDPRC; hematocrit equal 55.8%, volume equal 313 mL. and
white cell contamination equal 0.0 X 106 cells/u. Comparison
the value with SPSS statistics were found that the hematocrit,
volume and white blood cell contamination were not
different (P>0.01, P>0.05 and P>0.05, respectively). The
quality of LDPC(N=68); platelet contents equal 3.03 X 1011
cells/u, volume equal 255.8 mL. and white cell contamination
equal 0.0 X 106 cells/u. All values were not differ statistically
(P>0.05). The validation shown blood product specification,
Reveos NLR set blood performance data were; 20 units
test for volume and percent hematocrit Leuko-poor RBCs
in additive solution were found 200-350 mL, and 50-70 %
hematocrit and both ratio QC pass 100%. 19 units were
tested residual white blood cells in Leuko-poor RBCs found
95% has residual white blood cells less than 1.2X109 cells/
unit. All plasma product100% pass. 12 units tested of platelet
transfusable platelet (TPU) for volume, platelet yield and
residual white blood cells in TPU were found40-70 mL, all of
them platelet yield more than 5.5X1010 cells/unit (100% QC
pass) and residual white blood cells less than 0.2X109 cells/
unit (100% QC pass). Further, platelet recovery in leukocyte
depleted pooled platelet concentrates (4 units of interim
platelet unit per pool) 2 pool has describe in volume, platelet
yield, and residual were found; pool no.1 has 352.4 mL, 3.34
X1011 cells/units and 0.092x106 cells/unit. Pool no. 2 has
350.5 mL, 2.82x1011 cells/unit and 0.060x106 cells/unit.
Time per 4 units completed approximately 25 minutes.
Discussion and Conclusion: Council of Europe (CoE)
requirements; platelet yield must be more than 2.0 X1011
cells/unit, volume more than 40 mL per 60X109 of platelets
and residual white blood cells less than 1.0x106 cells/unit.
The benefits of process WB into Leuko-poor RBCs, platelet
and plasma in a single centrifuge cycle. Achieve consistent
and reliable blood performance criteria through automated
sedimentation and separation.
Biography:
E-mail:
bnhaw123@gmail.comPDF HTML