Proteolytic enzymes in the pathogenesis of the influenza virus
11th Annual Congress on Immunology
July 26-28, 2018 | Moscow, Russia
Valentina Divocha
Lugansk State Medical University, Ukraine
Posters & Accepted Abstracts : Timely Top Clin Immunol
Abstract:
Deproteinization of a flu virus is necessary for its penetration into a cell and this occurs for the account of trypsin-like proteinases of the host’s cell. We assumed that this enzyme has an important role in morphogenesis of flu virus and considerably defines its pathogenic and virulent properties. Objective: to study the changes of proteinase and inhibitor activities in the development of influenzal infection at white mice previously infected with flu A virus. Results: It has been being established that the level of trypsinlike proteinase and its inhibitor in the lungs and blood serum of not infected white mice were in balance at rather high level and did not change considerably during the whole period of supervision (6 days). At infection of white mice with virus of flu A/PR/8/34 (H1N1) there was a violation of proteinaseinhibitory balance. The most profound changes happened during the first hours after infection. There was the growth of proteinase activity and decrease of inhibitory activity. During the maximum accumulation of infectious titer of virus and its hemagglutinin, both proteinase and inhibitory activity was completely suppressed. The animals which didn’t perish for 5-6 days increase of inhibitory activity and decrease in proteinase took place. Conclusions: Increase of proteinase activity during the first hours after infection led to increase of infectious and hemagglutinating activity. The increase of inhibitory activity in 5-6 days after infection leads to some arresting of influenzal infection.
Biography:
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