Molecular characterization of a novel phage GW1 lysing Cronobacter
11th Annual Congress on Immunology
July 26-28, 2018 | Moscow, Russia
Haiyan Zeng, Wenjing He, Chengsi Li, Jumei Zhang, Na Ling, Liang Xue, Moutong Chen, Haoming Wu, Qingping Wu and Yu Ding
Guangdong Institute of Microbiology, China Jinan University, China
Scientific Tracks Abstracts : Timely Top Clin Immunol
Abstract:
Took Cronobacter strains as the host for phage isolation, we isolated some phages from Pearl River of Guangzhou, China. More importantly, we obtained a newly identified lytic Cronobacter phage GW1, which had a linear doublestranded DNA genome of 39,695 nucleotides with an average GC content of 53.18 %. Among the identified 49 open reading frames (ORFs), genes for rRNA, tRNA, antibiotic resistance, and virulence factors were not found in the phage genome. In BLASTn, the data revealed that the genome of phage GW1 had the highest identity of 94% to Citrobacter phage SH4, followed by 93% identity to Cronobacter phage Dev2. The morphology, genomic features, and phylogenetic analyses revealed that GW1 represented a new species of the T7virus genus. This novel lytic Cronobacter phage GW1 may provide an alternative phage therapy and biocontrol against Cronobacter.
Biography:
Haiyen Zheng did her Ph.D in Wuhan Institute of Virology , Chinese Academy of Sciences, Biochemistry and Molecular Biology, Bachelor of Medicine, Department of Medical Laboratory, Xiangya School of Medicine, Central South University. Pollution prevention and control and pathogenic mechanism of food-borne Cronobacter. Awards, honors and representation of academic groups at home and abroad: She received the National Scholarship, the First East Lake Academic Forum Excellent Exhibition Board Award, and the third prize of Hubei Excellent Academic Paper.
E-mail: zenghy@aliyun.com
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