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In vitro differentiation of mesenchymal stem cells from human umbilical cord Wharton’s jelly into functioning hepatocytes

Joint Event On World Congress on Tissue Engineering, Stem Cells and Regenerative Medicine & International Conference on Cell and Gene Therapy
March 14-15, 2019 | London, UK

Mervat Dawood

Mansoura University, Egypt

Posters & Accepted Abstracts : Biomed Res

DOI: 10.4066/biomedicalresearch-C1-026

Abstract:

Back ground: The human umbilical cord (UC) is non-invasive, primitive and abundant sources of mesenchymal stromal cells (MSCs) that have increasingly. Liver disease is a major cause of mortality and morbidity in Egypt. There are many inflammatory liver conditions for which treatments are not effective and often such patients will progress to end-stage liver disease and require liver transplantation. To prevent progression to endstage liver disease, mesenchymal stromal cell (MSC) therapies have been considered and shown to have potential in such liver diseases.

Objectives: The aim of our study was to investigate the in vitro differentiation of human umbilical cord Wharton’s jelly (HUMSC) into hepatocyte lineage.

Materials & methods: Human umbilical cord Wharton’s jelly (WJ) were separated by mixed explant & enzymatic method by use of trypsin. The time required for the primary culture range from 10-14 days. The isolated cells were characterized for expression of MSC-specific markers such as CD73, CD90 and CD105 & CD45. Also cells were counted by automated cell counter for stem cells (showing count, viability, cluster cells). After passage 4, the isolated cells induced to differentiate into hepatocyte-like cells by incubation in basal media with cocktail hepatocyte growth factors for 20 days.

Results: In vitro functional characterization of hepatocyte detectable by PAS staining for glycogen and immunofluorescent staining for albumin by anti-human albumin with FITC stain.

Conclusion: HU-MSC can differentiate into functional hepatocyte like cells & serve as a cell source for tissue engineering and cell therapy for hepatic tissues.

Biography:

E-mail:

mervatdaood@yahoo.com

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