Anti-rotavirus activity of rhubarb extract and emodin in vitro
10th American Pediatrics healthcare & Pediatric Infectious Diseases Congress
September 20-22, 2017 | Toronto, Canada
Yang Zhan-qiu, He Feng-lan, Liu Qiang, Wei Fei, Liu Yuan-yuan and Xiong Hai-rong
Wuhan University, China
Scientific Tracks Abstracts : Pediatric Healthcare & Pediatric Infections
The aim of this study was to evaluate the antiviral activity of rhubarb extract and emodin on rotavirus R709 strain in vitro. The titer and viral inhibition rate was evaluated for the antiviral activity of rhubarb extract and emodin in MA-104 cells infected with rotavirus(RV)R709 strain. Meanwhile, we carried out experiments through the three ways of preventive effect, virucidal effect, and antiviral biosynthesis effect against RV. The extracts from rhubarb showed significant inhibitory activity against rotavirus on MA-104 cells in a dose dependent manner when added at different stages of viral replication cycles. When added before, during or after viral infection, the 50% inhibitory concentration (IC50) was 101.08±1.57μgmL-1, 111.27±4.94μgmL-1 and 46.88±3.5μgmL-1 respectively. The therapeutic index (TI) of rhubarb extract was 3.13±0.13, 3.45±0.06 and 7.45±0.56 respectively. Rhubarb extract was highly active against rotavirus in vitro. However, emodin showed mild antiviral activity.
Yang Zhan-qiu has completed his MD from Wuhan University School of Medicine. He is the Director of Institute of Medical Virology, Wuhan University. He has published more than 150 papers in reputed journals and has been serving as an Editorial Board Member of repute.