Anti-inflammatory activity and modulate oxidative stress of Bucida buceras in Lipopolysaccharide- Stimulated RAW 264.7 macrophages and Carrageenan-Induced acute paw edema in rats
11th Annual Congress on Immunology
July 26-28, 2018 | Moscow, Russia
Simon Bernard Iloki Assanga
Rubio Pharma y Asociados, Mexico
Posters & Accepted Abstracts : Timely Top Clin Immunol
As oxi.dative stress is an important mediator that provoke or sustain inflammatory processes, we evaluated here the effects of Bucida buceras on inflammatory response in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and antiinflammatory effect and redox biomarkers in carrageenaninduced paw edema in rats. In a continuous effort to find more potent, non-toxic natural product inhibitors that suppress inflammation, the present study was carried out to analyzed the influence of aqueous extract on NO, TNF-α, IL-6 and IL- 1β in LPS-induced murine macrophages and paw thickness, NO, CPR, organoperoxide, oxidation protein and reducing power antioxidant in paw edema in rats. Treatment with Bucida buceras aqueous extract inhibited not only the protein (albumin) denaturation but also, in LPS-induced inflammatory response, including increased secretion of proinflammatory cytokines (IL-6 and IL-1β) and NO were inhibited by aqueous extract in a concentration-dependent manner. Furthermore, B. buceras suppressed significantly edema in a dose-dependent fashion in inflamed rat paws; decrease the C-reactive protein, lipid peroxidation levels (OT) and oxidation protein product and exerted strong reducing antioxidant power.