A novel predictor of leukemia transformation in Myelodysplastic syndrome patients
International Conference on Molecular Biology and Genetic Engineering
November 07-08, 2019 | Melbourne, Australia
EDA Hospital, Taiwan
Keynote : J Res Rep Genet
Myelodysplastic syndrome (MDS) is the disorder of hematopoietic stem cells. In MDS patients, leukemia progression is also associated with iron overload. Hydroxybutyrate dehydrogenase type 2 (BDH2) catalyzes the production of 2,5-dihydroxybenzoic acid (DHBA), an iron-binding component. In the present study, we assessed whether BDH2 can serve as a predictor for leukemia progression in MDS. The higher BDH2 expression (15%) group showed a greater risk for leukemia progression than the lower expression group (3.18%) (P=0.017). Additionally, we investigated the mechanism underlying the prognostic ability of BDH2 by using RNA interference–mediated-knockdown of BDH2 (BDH2-KD) in the acute leukemia cell line, THP1. Cell cycle analysis, surface markers, and special stain studies indicated that BDH2-KD induced differentiation and decreased the growth rate of THP1 cells, which was associated with the retardation of cell cycle. Under next-generation sequences analysis, we also found some candidate genes involving iron metabolism pathway contribute to leukemia transformation in MDS patients. Our study provides a foundation for further research on the role of BDH2 and iron metabolism in the pathogenesis of MDS.
Wen-Chi Yang has completed her MD Ph.D. from Kaohsiung medical University. She had 2 years postdoctoral studies from Harvard Medical School during 2007 to 2009 and half year postdoctoral studies from Massachusetts Institue of Technology after then. She is a hematology, medical oncology and hospice care specialist in Taiwan. She is the attending physician of EDA hospital. She is also the chief staff of hematology-oncology division and Biobank of EDA hospital. She is an assistant professor in Medical school, I-Shou University. She set up a molecular medicine lab in Yuan’s general hospital. Her interests are in hematology, molecular biology and stem cell biology field.
E-mail: [email protected]