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Journal of Timely Topics in Clinical Immunology | Volume 2
July 26-28, 2018 | Moscow, Russia
Immunology
11
th
Annual Congress on
Comparison of the Immunomodulatory effects of isolated stage 2 and stage 4 carcinoma associated
fibroblasts in mouse Breast Cancer model
Ladan Langroudi
Kerman University of Medical Sciences, Iran
Background:
studies have shown that the microenvironment
of solid tumors, specifically carcinoma associated
fibroblasts, affect the development and progression of
cancer. Additionally, it is responsible for immune evasion
and drug resistance of cancer cells. However, the crosstalk
between CAFs and the immune system is still unidentified.
Methods:
in this study, we characterized and compared the
phenotype and the immunomodulatory properties of stage 2
and 4 CAFs isolated from mammary tumor of Balb/C mouse.
Results: Flow cytometry of the surface marker panel showed
that stage 4 CAFs express significantly higher levels of HLA-
DR. Also, Higher levels of IL10, COX-2 and MMP9 enzyme
was also observed in stage 4 CAFs. Whereas in stage 2 CAFs
higher amounts of TFG-β and IDO expression was observed.
Conclusion: CAFs represent the supporting stroma of cancer.
They are known responsible for immune evading and growth
of cancer. Functional differences showed by their surface
markers, cytokine and enzyme production indicates to
induction of different microenvironments in their presence. The
discrepancy observed in cancer therapies may be attributed to
the influence of CAFs. Therefore, further studies are required
to fully elucidate the role of CAFs in various stages of cancer.
Speaker Biography
Ladan Langroudi did her Ph.D. in Medical Immunology, Tarbiat modares university, Tehran.
Master of Medical Immunology, Tarbiat modares university, Tehran. BSc in laboratory
sciences, Kerman University of Medical Sciences. Currently she is a professor of laboratory
sciences, Kerman University of Medical Sciences in the Department of Immunology.
e:
l.langroudi@kmu.ac.ir