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Journal of Timely Topics in Clinical Immunology | Volume 2
July 26-28, 2018 | Moscow, Russia
Immunology
11
th
Annual Congress on
Molecular characterization of a novel phage GW1 lysing
Cronobacter
Haiyan Zeng
1
, Wenjing He
1
, Chengsi Li
1
, Jumei Zhang
1
, Na Ling
1
, Liang Xue
1
, Moutong Chen
1
, Haoming Wu
1
, Qingping Wu
1
and
Yu Ding
2
1
Guangdong Institute of Microbiology, China
2
Jinan University, China
T
ook Cronobacter strains as the host for phage isolation,
we isolated some phages from Pearl River of Guangzhou,
China. More importantly, we obtained a newly identified
lytic Cronobacter phage GW1, which had a linear double-
stranded DNA genome of 39,695 nucleotides with an average
GC content of 53.18 %. Among the identified 49 open reading
frames (ORFs), genes for rRNA, tRNA, antibiotic resistance,
and virulence factors were not found in the phage genome.
In BLASTn, the data revealed that the genome of phage GW1
had the highest identity of 94% to Citrobacter phage SH4,
followed by 93% identity to Cronobacter phage Dev2. The
morphology, genomic features, and phylogenetic analyses
revealed that GW1 represented a new species of the T7virus
genus. This novel lytic Cronobacter phage GW1 may provide an
alternative phage therapy and biocontrol against Cronobacter.
Speaker Biography
Haiyen Zheng did her Ph.D in Wuhan Institute of Virology , Chinese Academy of
Sciences, Biochemistry and Molecular Biology, Bachelor of Medicine, Department of
Medical Laboratory, Xiangya School of Medicine, Central South University. Pollution
prevention and control and pathogenic mechanism of food-borne Cronobacter.
Awards, honors and representation of academic groups at home and abroad: She
received the National Scholarship, the First East Lake Academic Forum Excellent
Exhibition Board Award, and the third prize of Hubei Excellent Academic Paper.
e:
zenghy@aliyun.com