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allied
academies
Journal of Timely Topics in Clinical Immunology | Volume 2
July 26-28, 2018 | Moscow, Russia
Immunology
11
th
Annual Congress on
Anti-inflammatory activity and modulate oxidative stress of Bucida buceras in Lipopolysaccharide-
Stimulated RAW 264.7 macrophages and Carrageenan-Induced acute paw edema in rats
Simon Bernard Iloki Assanga
Rubio Pharma y Asociados, Mexico
A
s oxi.dative stress is an important mediator that provoke
or sustain inflammatory processes, we evaluated here
the effects of Bucida buceras on inflammatory response in
lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and anti-
inflammatory effect and redox biomarkers in carrageenan-
induced paw edema in rats. In a continuous effort to find more
potent, non-toxic natural product inhibitors that suppress
inflammation, the present study was carried out to analyzed
the influence of aqueous extract on NO, TNF-α, IL-6 and IL-
1β in LPS-induced murine macrophages and paw thickness,
NO, CPR, organoperoxide, oxidation protein and reducing
power antioxidant in paw edema in rats. Treatment with
Bucida buceras aqueous extract inhibited not only the protein
(albumin) denaturation but also, in LPS-induced inflammatory
response, including increased secretion of proinflammatory
cytokines (IL-6 and IL-1β) and NO were inhibited by aqueous
extract in a concentration-dependent manner. Furthermore, B.
buceras suppressed significantly edema in a dose-dependent
fashion in inflamed rat paws; decrease the C-reactive
protein, lipid peroxidation levels (OT) and oxidation protein
product and exerted strong reducing antioxidant power.
e:
ilokiasb@yahoo.com